EFFECTS OF DRUGS ON NUCLEIC ACIDS

这个项目的最终目标是了解药物与 以此为基础进行合理的设计 新药。荧光检测圆二色谱的新方法 (FDCD)将用于比较不同条件下的药物结合情况。FDCD 测量左、右激发的荧光强度的差异 圆偏振光。它为构象提供了一种灵敏的探针 围绕着一种荧光药物。由于荧光具有很高的检测率 灵敏度和特异度高，可用于低成本药物研究。 复杂系统中的浓度。简单与复杂中的药物结合 系统将进行比较。例如，体内的药物结合类似于 药物与溶液中的游离DNA结合？联合用药对血管紧张性贫血的影响 具有约束力也将被考虑。

The ultimate goal of this project is to understand the binding of drugs to nucleic acids in order to provide a foundation for the rational design of new drugs. The new method of fluorescence detected circular dichroism (FDCD) will be used to compare drug binding under various conditions. FDCD measures the difference in fluorescence intensity excited by left and right circularly polarized light. It provides a sensitive probe for conformation around a fluorescent drug. Since fluorescence has high detection sensitivity and specificity, it can be used to study drugs at low concentrations in complex systems. Drug binding in simple and complex systems will be compared. For example, is drug binding in vivo similar to drug binding to free DNA in solution? The effects of drug combinations on binding will also be considered.