COMPOSITE GRAFTS IN THE TREATMENT OF THERMAL INJURY

人同种异体中厚复合皮片的研制 真皮和自体表皮已显示出永久性的成功。 患者全层皮肤缺失的闭合。 该技术利用冷冻保存的真皮和水泡获取 自体表皮细胞不需要广泛或深部封闭 捐赠点。 目前的研究包括进一步开发分解的表皮 细胞制剂和输送系统，即最佳载体，以及 真皮制剂在移植物稳定性和安全性中的作用评价 抗原性。研究工作将针对以下方面： 1)完美的表皮细胞解聚方法，以增加覆盖率 潜力 2)建立最佳覆盖所需的小区密度 3)开发细胞递送系统，以允许易于应用和最大限度地 表面积保护 4)研究不同冷冻方法和冻存溶液对真皮的影响 可接受性 5)确定预防肌挛缩所需的真皮成分 6)检查真皮对移植物抗原性的贡献，包括 交叉样本分析 通过这些目标的实现，实现了复合材料的外延 移植实现较大表面积的完全和永久闭合 预计会有人员受伤。

A composite split thickness skin graft consisting of human allogeneic dermis and autologous epidermis has demonstrated success in permanent closure of full thickness skin losses in patients. The technique utilized cryopreserved dermis and blistered acquired autologous epidermal cells for closure without requiring extensive or deep donor sites. Current research involves further development of disaggregated epidermal cell preparations and delivery systems, i.e., optimal carriers, and evaluation of the role of dermal preparations in graft stability and antigenicity. Research efforts will be directed to: 1) Perfect method of disaggregation of epidermal cells to increase coverage potential 2) Establish cell density required for optimal coverage 3) Develop cell delivery systems to permit ease of application and maximum surface area protection 4) Investigate affect of cryopreservation methods and solutions on dermal acceptability 5) Determine dermal composition necessary to prevent contracture 6) Examine dermal contribution to graft antigenicity including cross-specimen analysis Through the accomplishment of these objectives extension of composite grafts to effect complete and permanent closure of larger surface area injuries is predicted.