PURIFICATION AND PROPERTIES OF CA2+ PUMPING ATPASE

钙泵结构与功能关系的研究 将继续红细胞质膜ATP酶。 Ca 2+泵 是从心脏，其他肌肉， 肾、脑、肠上皮和其他几种细胞类型;所有 这些细胞类型含有质膜Ca 2+泵ATP酶， 类似于红细胞。 这种酶由一个单一的 多肽链，Mr = 140，000;有可能获得纯的， 从人红细胞中提取合理量。 我们提出的研究设计 创建酶的一级结构图，并定位 这种酶的各种功能。 研究还将进行 通过脂质对酶的调节， 磷脂酰肌醇循环和蛋白水解。

Studies on the relationship of structure and function of the Ca2+ pumping ATPase of erythrocyte plasma membranes will be continued. This Ca2+ pump is the archetype for plasma membrane Ca2+ pumps from heart, other muscle, kidney, brain, intestinal epithelium and several other cell types; all of these cell types contain plasma membrane Ca2+ pumping ATPases closely resembling that from the erythrocyte. This enzyme consists of a single polypeptide chain, Mr = 140,000; it is possible to obtain it pure and in reasonable quantity from human erythrocytes. We propose studies designed to create a map of the primary structure of the enzyme, and to locate the various functions of this enzyme on the map. Studies will also be carried out on the regulation of the enzyme by the lipids of the phosphatidylinositol cycle and by proteolysis.