HIGH FIELD FT MASS SPECTROMETRY FOR BIOMOLECULES

要求继续支持一个项目，该项目将展示 傅里叶变换质谱学在高分辨率中的适用性 质量在1,500-20,000道尔顿离子的精确质量分析 射程。这将使用(1)激光解吸；(2)铯 离子轰击；(3)快原子轰击；(4)热喷雾技术 样品挥发与差动泵浦的双池联用 傅里叶变换质谱仪和8T磁铁。预计 在m/z 20,000或以上的前所未有的高质量分辨率将是 演示了。此外，相对灵敏度、准确度和 将根据将获得的结果来确定解决方案 在ABZ-1HF双高性能VG仪器上使用相同的样品 聚焦质谱仪。方法论的适用性 发展到双聚合物分析(例如，多肽和多糖)将是 调查过了。

Continued support is requested for a project which will demonstrate the applicability of Fourier transform mass spectrometry to high resolution accurate mass analysis of ion with masses in the 1,500-20,000 dalton range. This will be accomplished using (1) laser desorption; (2) cesium ion bombardment; (3) fast atom bombardment; (4) thermospray techniques for sample volatilization in conjunction with a dual cell differentially-pumped Fourier transform mass spectrometer and an 8T magnet. It is expected that unprecedented high mass resolution at m/z 20,000 or above will be demonstrated. Furthermore, the relative sensitivity, accuracy, and resolution will be determined in comparison with results to be obtained with the same samples on a high performance VG Instruments ABZ-1HF double focussing mass spectrometer. The applicability of the methodologies developed to bipolymer analyses (e.g., polypeptides and polysugars) will be investigated.