MAMMALIAN CELL SURFACE GENETICS AND BIOCHEMISTRY

动物细胞的细胞表面被认为参与了许多 多细胞生物体独有的重要生物过程。它是 通常假设细胞表面，要么基于其详细的化学成分， 拓扑重排或通过包括组装、周转和 与其他细胞器的动态相互作用，在调节中起着关键作用 正常和恶性肿瘤中细胞增殖和细胞间相互作用的研究 细胞。 该研究项目的目的是阐明细胞的结构和功能。 哺乳动物细胞表面通过分离和生化鉴定 体细胞突变体。更具体地说，我们计划；a.分离和 鉴定CHO突变体对细胞毒素的抗性，包括植物凝集素和 微生物毒素；B.分离和鉴定改变的CHO突变体 细胞骨架-膜复合体的结构和功能完整性 据信与表面调节组件相互作用的化学物质； 确定这些突变的生理后果。 通过确定细胞内变化之间的因果关系 表面成分和生长控制的丧失，我们可能会有更好的洞察力 从细胞和分子水平探讨肿瘤的发病机制。

The cell surface of animal cells has been implicated to participate in a number of important biological processes unique to multicellular organisms. It is generally assumed that the cell surface, either based on its detailed chemistry, topological rearrangements or by processes including assembly, turnover and dynamic interactions with other organelles, plays a key role in the regulation of cellular proliferation and in cell-cell interaction in normal and malignant cells. The aim of the research project is to elucidate the structure and functions of mammalian cell surface through the isolation and biochemical characterization of somatic cell mutants. More specifically, we plan; a. to isolate and characterize CHO mutants resistant to cytotoxins, including plant lectins and microbial toxins; b. to isolate and characterize CHO mutants altered in the structural and functional integrity to cytoskeleton-membrane complex by using chemicals believed to interact with surface modulating assemblies; and c. to ascertain the physiological consequences of these mutations. By ascertaining the cause-effect relationship between the alteration in cell surface component and the loss of growth control, we may gain a better insight into the pathogenesis of neoplasia at the cellular and molecular level.