INVESTIGATIONS OF THE CHORISMATE PATHWAY

拟议的研究计划的目标是提供一个详细的 对芳香族氨基酸生物合成与生长的认识 细菌中分枝酸的影响因素。这些生物合成途径 初级新陈代谢是植物界独有的。……的生物合成 酪氨酸、邻氨基苯甲酸酯(色氨酸的前体)、对氨基苯甲酸酯和 异甲酸酯(2，3-二羟基苯甲酸酯和间-羧基芳香族的前体 氨基酸)是主要感兴趣的。 用于研究的多种物质的合成 酶催化的途径将被完成。这些物质将是 用于阐明邻氨基苯甲酸酯的机理细节和 对氨基苯甲酸酯的生物合成。在非合作的努力中，物质 将被用于阐明酪氨酸和酪氨酸的机制细节 从分枝酸生物合成异分枝酸。 该计划的最终目标是发现类似于 生物合成中间体将成为有用的抗菌剂或 选择性抑制体内靶标酶的抗真菌药物 分支酸途径。

The goal of the proposed research program is to provide a detailed understanding of the biosynthesis of aromatic amino acids and growth factors from chorismate in bacteria. These biosynthetic pathways of primary metabolism are unique to the plant kingdom. The biosynthesis of tyrosine, anthranilate (precursor to tryptophan), p-aminobenzoate, and isochorismate (precursor to 2,3-dihydroxybenzoate and m-carboxyaromatic amino acids) are of principle interest. The synthesis of numerous substances for investigation of the enzyme-catalyzed pathways will be accomplished. These substances will be utilized to elucidate mechanistic details of anthranilate and p-aminobenzoate biosynthesis. In noncollaborative efforts the substances will be utilized to elucidate the mechanistic details of tyrosine and isochorismate biosynthesis from chorismate. A final objective of the program is the discovery of analogs of biosynthetic intermediates that will be useful antibacterial agents or antifungal agents by selective inhibition of target enzymes in the chorismate pathway.