COMPOSITE GRAFTS IN THE TREATMENT OF THERMAL INJURY

一种由人同种异体皮肤组成的复合中厚皮肤移植物， 真皮和自体表皮已经证明了在永久性 闭合患者的全层皮肤缺损。 该技术利用冷冻保存的真皮和获得的 自体表皮细胞用于闭合，而不需要广泛或深的 供体部位。 目前的研究涉及进一步开发分解的表皮 细胞制剂和递送系统，即，最佳运营商，以及 真皮制剂在移植物稳定性中的作用的评价， 抗原性 研究工作将针对： 1)完美的表皮细胞解聚方法，以增加覆盖率 潜在 2)建立最佳覆盖所需的小区密度 3)开发细胞输送系统，使其易于应用， 表面保护 4)探讨冷冻方法和溶液对真皮的影响 接受性 5)确定防止挛缩所需的真皮成分 6)检查皮肤对移植物抗原性的贡献，包括 交叉样本分析 通过这些目标的实现， 移植物以实现较大表面积的完全和永久闭合 伤害是可以预测的。

A composite split thickness skin graft consisting of human allogeneic dermis and autologous epidermis has demonstrated success in permanent closure of full thickness skin losses in patients. The technique utilized cryopreserved dermis and blistered acquired autologous epidermal cells for closure without requiring extensive or deep donor sites. Current research involves further development of disaggregated epidermal cell preparations and delivery systems, i.e., optimal carriers, and evaluation of the role of dermal preparations in graft stability and antigenicity. Research efforts will be directed to: 1) Perfect method of disaggregation of epidermal cells to increase coverage potential 2) Establish cell density required for optimal coverage 3) Develop cell delivery systems to permit ease of application and maximum surface area protection 4) Investigate affect of cryopreservation methods and solutions on dermal acceptability 5) Determine dermal composition necessary to prevent contracture 6) Examine dermal contribution to graft antigenicity including cross-specimen analysis Through the accomplishment of these objectives extension of composite grafts to effect complete and permanent closure of larger surface area injuries is predicted.