INVESTIGATIONS OF THE CHORISMATE PATHWAY

该研究计划的目标是提供一个详细的 了解芳香族氨基酸的生物合成和生长 细菌中分支酸的因子。 这些生物合成途径 初级代谢是植物界所独有的。 的生物合成 酪氨酸、邻氨基苯甲酸（色氨酸的前体）和异分支酸 （2，3-二羟基苯甲酸和间羧基芳香族氨基酸的前体）是 的主要利益。 为了研究酶而合成了许多物质- 将完成催化途径。 这些物质将用于 与C.T.教授合作沃尔什（哈佛医学院）， 阐明邻氨基苯甲酸酯，对氨基苯甲酸酯， 异分支酸和2，3-二羟基苯甲酸生物合成。 在非合作 这些物质将被用来阐明 从分支酸生物合成酪氨酸和异分支酸的细节。 该计划的最终目标是发现类似物， 将是有用的抗菌剂的生物合成中间体或 通过选择性抑制靶酶的抗真菌剂 分支酸途径

The goal of the proposed research program is to provide a detailed understanding of the biosynthesis of aromatic amino acids and growth factors from chorismate in bacteria. These biosynthetic pathways of primary metabolism are unique to the plant kingdom. The biosynthesis of tyrosine, anthranilate (precursor to tryptophan), and isochorismate (precursor to 2,3-dihydroxybenzoate and m-carboxyaromatic amino acids) are of principle interest. The synthesis of numerous substances for investigation of the enzyme- catalyzed pathways will be accomplished. These substances will be utilized in collaboration with Professor C.T. Walsh (Harvard Medical School) to elucidate mechanistic details of anthranilate, p-aminobenzoate, isochorismate, and 2,3-dihydroxybenzoate biosynthesis. In noncollaborative efforts the substances will be utilized to elucidate the mechanistic details of tyrosine and isochorismate biosynthesis from chorismate. A final objective of the program is the discovery of analogs of biosynthetic intermediates that will be useful antibacterial agents or antifungal agents by selective inhibition of target enzymes in the chorismate pathway.