DEVELOPMENT OF BIOSELECTIVE VOLTAMMETRIC SENSORS

本研究的目标是发明、设计、研究和测试 适用于临床的有效伏安生物传感器 治疗监测 主要战略将继续是 分析物在工作表面上的优先积累 电极表面，然后通过伏安定量的 表面结合物种。 分析物预浓缩将 通过选择性连接，通过共价或离子- 在修饰电极上的交换反应，或优先 吸附在普通电极上。 聚合物涂层， 可控渗透率，将被评估，以提高 传感器的选择性和稳定性。 传感方案， 各种临床上重要的分析物，特别是治疗 毒品，将被调查。 区别于 所提出的传感器是固有的灵敏度（ 预浓缩步骤和先进的伏安技术）， 选择性（基于定义的化学反应和 聚合物涂层的区别性），简单， 经济性、速度和小型化。 调查将涵盖 这种生物传感器的基本和实际方面， 包括对传感器行为的详细研究， 优化运营条件，竞争， 临床试验的结果方案。 的具体问题 包括高灵敏度的吸附汽提 抗癌药物和微量元素的测量方案， 适当调整（修改）电极， 用作预浓缩表面， 内源性生物材料、联合给药药物，或 主要代谢物，基于 复合材料和双层聚合物组件以及混合碳 糊剂、表面条件和电极处理的影响 程序上的吸附溶出响应，并重现 再生新的传感器表面。 通过适当解决 上述问题将获得显著的分析优势， 以及伏安生物传感器在临床上的实用性 工具将更接近实现。

The goal of this research is to invent, design, study and test effective voltammetric biosensors suitable for use in clinical therapeutic monitoring. The principal strategy will continue to be the preferential accumulation of the analyte onto the working electrode surface, followed by voltammetric quantitation of the surface-bound species. Analyte preconcentration will be accomplished by selective attachment, via covalent or ion- exchange reactions at modified electrodes, or preferential adsorption at ordinary electrodes. Polymeric coatings, with controllable permeability, will be evaluated for improving the selectivity and stability of the sensors. Sensing schemes for a variety of clinically-significant analytes, particularly therapeutic drugs, will be investigated. The features that distinguish the proposed sensors are inherent sensitivity (combination of a preconcentration step and advanced voltammetric techniques), selectivity (based on defined chemical reactions and discriminative properties of polymeric coatings), simplicity, economy, speed, and miniaturization. The investigation will cover both the fundamental and practical aspects of such biosensors, including detailed studies of the sensor behavior, systematic optimization of operational conditions, and competition and clinical tests of the resulting schemes. Specific issues to be addressed include highly sensitive adsorptive stripping measurement schemes for anticancer drugs and trace elements, appropriate tailoring (modification) of electrodes for effective use as preconcentrating surfaces, interferences due to endogeneous biological materials, coadministrated drugs, or principal metabolites, new modified electrodes based on composite and bilayer polymer assemblies and mixed carbon paste, effects of surface conditions and electrode treatment procedures on the adsorptive stripping response, and reproducible regeneration of a fresh sensor surface. By properly addressing the above issues significant analytical advantages will be achieved, and the utility of voltammetric biosensors as practical clinical tools will come much closer to realization.