MOLECULAR REGULATION OF IMMUNOGLOBULIN GENE EXPRESSION

小鼠12号染色体上编码的基因是受调控的 免疫系统的发育。其中包括重免疫球蛋白。 链基因本身，以及T细胞的标志和至少一个 肿瘤细胞的一类。这些基因已经被广泛研究，不仅是 因为它们与免疫功能相关，但因为它们提供了一种 功能强大的发育相关分子遗传分析模型系统 哺乳动物系统中的过程。在这里，我们建议结合使用 重组DNA技术与体细胞遗传学系统 确定小鼠12号染色体的额外遗传标记，并开始 定义这些基因的可能功能。具体来说，重组DNA 技术将被用来克隆来自于 染色体。用这些片段定义的DNA多态将被用于 扩展并填写染色体的孟德尔图和物理图。 筛选杂交技术将被用来鉴定这些克隆的DNA 在特定组织和特定组织中在RNA水平上表达的片段 淋巴细胞发育的特定阶段。

Genes encoded on mouse chromosome 12 are central to the regulated development of the immune system. These include the immunoglobulin heavy chain genes themselves, as well as markers of T cells and at least one class of tumor cells. These genes have been widely studied, not only because of their relevance to immune function, but because they provide a powerful model system for the molecular genetic analysis of developmental processes in a mammalian system. Here, we propose to use a combination of recombinant DNA technology with somatic cell genetics to systematically define additional genetic markers of mouse chromosome 12, and to begin to define possible functions for these genes. Specifically, recombinant DNA techniques will be used to clone genomic DNA fragments derived from the chromosome. DNA polymorphisms defined with these fragments will be used to extend and fill in the Mendelian and physical maps of the chromosome. Filter hybridization techniques will be used to identify these cloned DNA fragments that are expressed at the RNA level in particular tissues and at particular stages in lymphocyte development.