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STRUCTURE OF DNA--SPECTROSCOPIC STUDIES

STRUCTURE OF DNA--SPECTROSCOPIC STUDIES
DNA 的结构——光谱研究
批准号:
3288656
负责人:
JOHN C SUTHERLAND
金额:
$11.38万
依托单位国家:
美国
项目类别:
财政年份:
1986
资助国家:
美国
项目状态:
已结题
起止时间:
1986-01-01 至 1991-12-31

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中文摘要
翻译
DNA的三维结构受碱基序列的影响,
英文摘要
The three dimensional structure of DNA as influenced by base sequence, metal ions, chemical carcinogens and other agents will be studied by vacuum-ultraviolet circular dichroism (VUV-CD) and time resolved fluorescence. The electronic states and transitions of the DNA bases, understanding of which is crucial to the interpretation of CD spectra, will be studied using vacuum-ultraviolet magnetic circular dichroism (VUV-MCD) and fluorescence polarization spectroscopy. A major goal is understanding why VUV-CD is a better indicator of the helical structure of, and more sensitive to the hydrogen bonding between DNA strands than is conventional, longer wavelength CD. The VUV-CD, VUV-MCD, fluorescence polarization and time-resolved fluorescence experiments will be performed at the National Synchrotron Light Source.
期刊论文(7)
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会议论文
Fluorescence spectral characteristics and fluorescence decay profiles of covalent polycyclic aromatic carcinogen-DNA adducts.
共价多环芳香族致癌物-DNA 加合物的荧光光谱特征和荧光衰减曲线。
DOI: 10.1007/978-1-4684-8041-2_21
发表时间: 1989
期刊: Basic life sciences
影响因子: --
作者: [Kim,SK, Geacintov,NE, Zinger,D, Sutherland,JC]
通讯作者: Sutherland,JC
A.U and G.C base pairs in synthetic RNAS have characteristic vacuum UV CD bands.
合成 RNAS 中的 A.U 和 G.C 碱基对具有特征真空 UV CD 谱带。
DOI: 10.1002/bip.360290205
发表时间: 1990
期刊: Biopolymers
影响因子: 2.9
作者: [Johnson,KH, Gray,DM, Morris,PA, Sutherland,JC]
通讯作者: Sutherland,JC
Vacuum UV CD spectra of homopolymer duplexes and triplexes containing A.T or A.U base pairs.
含有 A.T 或 A.U 碱基对的均聚物双链体和三链体的真空 UV CD 光谱。
DOI: 10.1093/nar/19.9.2275
发表时间: 1991
期刊: Nucleic acids research
影响因子: 14.9
作者: [Johnson,KH, Gray,DM, Sutherland,JC]
通讯作者: Sutherland,JC
Unidirectional pulsed-field electrophoresis of single- and double-stranded DNA in agarose gels: analytical expressions relating mobility and molecular length and their application in the measurement of strand breaks.
琼脂糖凝胶中单链和双链 DNA 的单向脉冲场电泳:与迁移率和分子长度相关的分析表达式及其在链断裂测量中的应用。
DOI: 10.1016/0003-2697(87)90427-1
发表时间: 1987
期刊: Analytical biochemistry
影响因子: 2.9
作者: [Sutherland,JC, Monteleone,DC, Mugavero,JH, Trunk,J]
通讯作者: Trunk,J
共 6 条
    STORAGE PHOSPHOR IMAGING SYSTEM
    NEW APPROACHES TO DNA MAPPING--ELECTRONIC IMAGING
    NEW APPROACHES TO DNA MAPPING--ELECTRONIC IMAGING
    NEW APPROACHES TO DNA MAPPING--ELECTRONIC IMAGING
    海外基金