STRUCTURE OF DENITRIFICATION ENZYMES

拟议的项目将确定酶的结构元素。 负责异化反硝化过程中亚硝酸盐的还原。质子 核磁共振波谱将被用来确定结构 关于催化中心的血红素基团，包括表征 电子态。共振拉曼光谱将用于 研究了催化位的振动态。完全相同的 该酶的修复基的化学结构将是 通过从完整的酶和结构中提纯这些物质来确定 通过核磁共振、振动、光学和 质谱学。氮氧化物与全细胞的相互作用 还将接受检查。靶标酶系统代表了 电子传递酶和蛋白质的总类。它的研究将 促进对这门课的基本理解，即 负责所有细胞的能量生产和呼吸。

The proposed project will determine structural elements for the enzyme responsible for nitrite reduction in dissimilatory denitrification. Proton nuclear magnetic resonance spectroscopy will be used to determine structure about the catalytic site heme groups, including the characterization of electronic states. Resonance raman spectroscopy will be used to investigate the vibrational states of the catalytic sites. The exact chemical structure of the prosthetic groups of the enzyme will be determined by purification of these from the intact enzyme and a structural determination through nuclear magnetic resonance, vibrational, optical, and mass spectroscopy. The interactions of oxides of nitrogen with whole cells will also be examined. The target enzyme system is representative of the general class of electron transport enzymes and proteins. Its study will promote the understanding on a fundamental level of this class which is responsible for energy production and respiration in all cells.