ROLE OF THE GENE PRODUCTS IN YEAST PRE-MRNA SPLICING

酵母基因RNA 2至RNA 11是酵母细胞生长所必需的。 信使RNA中的核前体的加工。 温度 这些基因中的敏感隐性突变导致 在未拼接的非允许温度下的累积 含内含子的mRNA前体。 证据的积累 这些RNA基因直接参与剪接。 的RNA 存在于酵母细胞核中， 这些RNA基因在体外剪切提取物。 一 遗传学、分子生物学和生物化学相结合的方法 将被用来确定RNA 2，RNA 3， RNA 4和SRN 2基因在酵母mRNA剪接中的作用。 使用克隆的 RNA基因和针对RNA基因产物的抗血清 以下问题将得到解决：（1）RNA基因 剪接复合体-剪接体或snRNP中的产物 还是HnRNPs (2)还有哪些分子与RNA相互作用 基因产物，它们是否参与mRNA剪接？ (3)如何 RNA 3和RNA 4，SRN 2和RNA 2基因是否与 彼此？ (4)的组装和功能中的哪些步骤 剪接复合体需要RNA基因的功能吗？

The yeast genes RNA2 through RNA11 are necessary for the processing of nuclear precursors in messenger RNA. Temperature sensitive recessive mutations in these genes result in the accumulation at the nonpermissive temperature of unspliced intron-containing precursors of mRNA. Evidence is accumulating that these RNA genes are directly involved in splicing. The RNA are in the yeast nucleus and temperature sensitive mutations in these RNA genes inactivate splicing extracts in vitro. A combined genetic, molecular biological, and biochemical approach will be taken to determine the function of the RNA2, RNA3, RNA4, and SRN2 genes in yeast mRNA splicing. Using the cloned RNA genes and antisera directed against the RNA gene products the following questions will be addressed: (1) Are the RNA gene products in the splicing complex - the spliceosome, or in snRNPs or HnRNPs? (2) What other molecules interact with the RNA gene products, and are they involved in mRNA splicing? (3) How do the RNA3 and RNA4, and SRN2 and RNA2 genes interact with each other? (4) Which steps in the assembly and function of the splicing complex require the function of RNA genes?