SEQUENCE ANALYSIS OF THE BACTERIAL FLAGELLAR MOTOR

该提案旨在了解生物学 能量转换、转换、结构和 组装，如细菌鞭毛马达中所例示的。 狂野- 编码组分的选定基因的类型和突变等位基因 鼠伤寒沙门氏菌的鞭毛马达 将按顺序排列，以确定 蛋白质，以及这些区域中的氨基酸，最 对组装和功能的各个方面至关重要;建议 建立在大量现有的详细的经典遗传学基础上， 信息. 主要有三个主题：（一）分析五个 基因（flaAII.2，flaQ，flaN，motA和motB），已知是 参与能量转换（质子势的转换 转换成机械旋转功）和切换（ （二）对专利权的保护; 鞭毛蛋白、钩蛋白和钩相关蛋白的结构基因 试图发现它们识别的基础 通过鞭毛特异性的输出途径，而不是通过 常规的N-末端信号肽依赖性途径;（iii） 分析鞭毛蛋白基因中的突变等位基因， 组装失败或组装成异常螺旋 多晶型物，以便鉴定重要的残基 亚基间的准等价相互作用 灯丝的螺旋度 随着假说的出现， 突变体序列分析，它们将通过产生位点- 定向突变

The proposal is directed to an understanding of biological processes of energy transduction, switching, structure, and assembly, as exemplified in the bacterial flagellar motor. Wild- type and mutant alleles of selected genes coding for components of the flagellar motor of the bacterium Salmonella typhimurium will be sequenced with a view to determining which regions of the proteins, and which amino acids within those regions, are most critical for various aspects of assembly and function; the proposal builds on a large existing body of detailed classical genetic information. There are three main topics: (i) analysis of the five genes (flaAII.2, flaQ, flaN, motA, and motB) that are known to be involved in energy transduction (conversion of proton potential into mechanical work of rotation) and switching (between counterclockwise and clockwise rotation); (ii) comparison of the structural genes for flagellin, hook protein, and hook-associated proteins in an attempt to discover the basis for their recognition by a flagellar-specific export pathway rather than by the conventional N-terminal signal peptide dependent pathway; (iii) analysis of mutant alleles in the flagellin gene that give rise to failure of assembly or to assembly into abnormal helical polymorphs, with a view to identifying residues that are important in the quasi-equivalent inter-subunit interactions that generate the helicity of the filament. As hypotheses emerge from the mutant sequence analysis, they will be tested by generating site- directed mutations.