THE ROLE OF ALPHA-LACTALBUMIN IN LACTOSE BIOSYNTHESIS

这项研究的最终目标是了解分子细节 半乳糖基转移酶与细胞因子的相互作用 “修饰”蛋白，α-乳清蛋白。由于一般糖基转移是通过 这种酶存在于雄性和雌性物种的其他器官中，它是 当(&乳清蛋白)是生物调节的一个重要例子 仅在哺乳期乳腺中分泌，以改变 专门用于乳糖合成的系统。 这项研究的长期目标是(1)表征、分配和 在α-乳清蛋白分子上定位特定的阳离子结合区域和其他区域 在溶液中，(2)表征、描述和绘制底物(UDPga) 半乳糖上的供体中心、金属中心(S)和(碳水化合物)受体中心 转移酶；以及(3)描述涉及到的残基和相互作用 A-乳清蛋白-半乳糖转移酶(“乳糖合成酶”)复合体， 即a-乳清蛋白在乳糖生物合成中的作用。这些研究将 涉及到电子自旋共振、核磁共振、光谱分析、荧光光谱等技术。 生化和酶学方法。

The ultimate goal of this research is to understand in molecular detail the interactions involved between the enzyme galactosyl transferase and the "modifier" protein, alpha-lactalbumin. Since general glycosyl transfer by this enzyme occurs in both male and female species in other organs, it is an important example of biological regulation when (&-lactalbumin is secreted only in lactating mammary gland to alter the specificity of the system specifically for lactose synthesis. The long term goals of this research are to (1) characterize, assign and map specific cation binding and other regions on the a-lactalbumin molecule in solution, (2) to characterize, describe and map the substrate (UDPgal) donor site, metal site(s) and (carbohydrate) acceptor site on galactosyl transferase; and (3) to describe the residues and interactions involved in the a-lactalbumin-galactosyl transferase ("lactose synthase") complex, i.e., the role of a-lactalbumin in lactose biosynthesis. These studies will involve the techniques of ESR, NMR, photo-CIDNP, fluorescence as well as biochemical and enzymological methods.