Sequencing and assembling microbial community metagenomes in real-time

该项目旨在利用纳米孔测序直接从环境样本中组装宏基因组。元基因组学的短读方法不能从环境样本中组装混合基因组，因此将重点放在描述存在哪些物种和基因上。长读纳米孔测序能够在一个样品中组装多个物种的全基因组。在水和土壤等重要资源中组装完整的基因组，将有助于更深入地了解这些资源微生物群落中遗传物质的动态、变异和转移，以及管理微生物多样性的策略，并提高这些资源的生产力和长期可持续性。

This project aims to assemble metagenomes directly from environmental samples using nanopore sequencing. Short-read approaches to metagenomics cannot assemble mixed genomes from an environmental sample, so focus on describing which species and genes are present. Long-read nanopore sequencing enables the assembly of full genomes of multiple species in a sample. Assembling complete genomes in important resources such as water and soil should lead to deeper understanding of the dynamics, variation and transfer of genetic material within these resources’ microbial communities, strategies to manage microbial diversity, and improved productivity and long-term sustainability for these resources.