ORGANIC SECONDARY ION IMAGING

本研究的目的是开发一种方法来确定 目标有机化合物在生物组织中的空间分布。 这样的能力将使快速分析组织成为可能， 毒素和药物的本地化，很可能，他们的 代谢物。 二次离子质谱（西姆斯）将用于 获得组织的有机离子显微照片。 空间分辨率， 可以达到的将由二次的实际产量决定 离子，即检测到的分析物特征二次离子的数量 每个入射初级离子。 将通过以下方式优化仪器功能： 离子光学设计标准严格应用于整个次级 离子质谱仪 原位化学预处理的方法 样品将被开发用于增强特征二次离子 从选定的分析物发射。 初级离子束种类的选择和 将研究优化二次离子产率的条件。 串联 将采用质谱（MS/MS），以便特征离子可以 除了它们的质荷比， 如目前在西姆斯中所实施的。 数据将使用现有的 IBM PC/XT数据系统，具有离线数据处理和图像处理功能 由DEC MicroVAX计算机执行。

The objective of this research is development of a method to determine spatial distributions of targeted organic compounds in biological tissue. Such a capability would make possible rapid analysis of tissue for localization of toxins and pharmaceuticals, and quite possibly, their metabolites. Secondary ion mass spectrometry (SIMS) will be employed to obtain organic ion micrographs of the tissue. The spatial resolution which can be attained will be determined by the practical yield of secondary ions, that is, the number of analyte-characteristic secondary ions detected per incident primary ion. Instrumental capabilities will be optimized by rigorous application of ion optical design criteria to the entire secondary ion mass spectrometer. Methods of in situ chemical pretreatment of the sample will be developed for enhancing characteristic secondary ion emission from selected analytes. Choice of primary ion beam species and conditions will be investigated to optimize secondary ion yield. Tandem mass spectroscopy (MS/MS) will be employed so that characteristic ions can be resolved by their structure, in addition to their mass-to-charge ratios, as is currently practiced in SIMS. Data will be obtained using an existing IBM PC/XT data system, with off-line data manipulation and image processing performed by a DEC MicroVAX computer.