MESSENGER RNA DECAY IN MAMMALIAN CELLS

这些研究将集中在阐明原癌基因的mrna。 哺乳动物细胞的降解。这些研究的目标将是 为了确定导致这种病毒的RNA结构特征 生长因子诱导的c-fos原癌基因的易感性 文字记录确定这些不稳定的机制(S) 决定因素在mRNA衰变中的作用，并表征 执行这一过程的细胞质因子。初级阶段 要采用的实验方法将涉及创建定义的 C.fos基因的体外突变和融合，介绍 这些改变后的基因结构转化为小鼠成纤维细胞，以及 分析产生的mRNA转录本在体内的衰变情况。 细胞mRNA降解因子的表征将涉及 牙齿检测不同剂量处理后细胞c-fos基因表达的变化 刺激细胞生长和净化这些降解的试剂 来自细胞质提取液的因子。这些研究的结果 应该提高我们对基因的一个基本方面的认识 目前还很难理解的表达方式，应该 有助于我们的长期目标，即确定基本的 管理真核生物中信使核糖核酸衰变的原则。 此外，这些知识最终应该对 了解肿瘤发生的生物学机制。

These investigations will focus on elucidating proto-oncogene mRNA degradation in mammalian cells. The goal of these studies will be to identify the RNA structural features responsible for the liability of the growth factor-inducible c-fos proto-oncogene transcript to determine the mechanism(s) by which these instability determinants function in mRNA decay, and to characterize the cytoplasmic factors that carry out this process. The primary experimental approach to be employed will involve creating defined mutations and fusions of the c. fos gene in vitro, introducing these altered genetic constructs into mouse fitroblasts, and analyzing the decay of the resulting mRNA transcripts in vivo. Characterization of cellular mRNA degradation factors will involve tooth examining c-fos mRNA decay in cells treated with various agents that stimulate cell growth and purifying these degradation factors from cytoplasmic extracts. The results of these studies should enhance our knowledge of a fundamental aspect of gene expression that presently is poorly understood and should contribute to our long-term objective of defining the basic principles that govern mRNA decay in eukaryotic organisms. Moreover, this knowledge should ultimately be of value to understanding biological mechanisms of oncogenesis.