PROPERTIES OF UTERINE EPITHELIUM RELATED TO IMPLANTATION

提出的研究将检查子宫上皮的特性 质膜与激素调节的获取相关 囊胚贴壁和着床的接受条件。 孕酮和雌二醇诱导的上皮细胞膜改变 性质(表面电荷，特定的凝集素结合，糖萼结构， 已经为兔子宫定义了糖蛋白/多肽图谱)。 针对阶段特异性膜蛋白的单特异性抗血清将是 产生并用作上皮细胞接受状态的探针 在体内和体外模型系统中。漂浮胶原胶原胶原凝胶在医疗中的应用 子宫上皮细胞的培养允许细胞的保留 活体观察表型。类固醇激素和激素的作用 子宫内膜间质细胞对上皮细胞分化的影响 本文将对细胞膜感受性标志的出现进行研究。 文化体系。膜组件在植物生长发育中的功能作用 将使用细胞黏附分析来探索与滋养层细胞的相互作用 “接受型”和“非接受型”上皮细胞培养。这项研究将 检验子宫上皮是进入子宫的屏障 滋养层细胞在非接受性子宫中的附着 激素介导的上皮细胞质膜的变化对 在卵子植入时允许粘连和附着。澄清 子宫和胚胎之间同步性发育的机制是 对理解植入过程至关重要，因此具有相关性 人类和其他动物物种的生育调节。

The research proposed will examine properties of the uterine epithelial plasma membrane which correlate with the hormone-regulated acquisition of the receptive condition to blastocyst adhesion and implantation. Progesterone- and estradiol-induced alterations in epithelial membrane properties (suface charge, specific lectin binding, glycocalyx structure, glycoprotein/polypeptide profile) have been defined for the rabbit uterus. Monospecific antisera against stage-specific membrane proteins will be produced and used as probes for the receptive state of the epithelium both in vivo and in an in vitro model system. Use of floating collagen gels for culture of uterine epithelial cells allows for retention of the cellular phenotype observed in vivo. The effects of steroid hormones and of endometrial stromal cells on the differentiation of epithelium and on the appearance of membrane markers of receptivity will be studied in this culture system. The functional role of membrane components in the interaction with trophoblast will be explored using cell adhesion assays on "receptive" and "non-receptive" epithelial cultures. This research will test the hypothesis that the uterine epithelium is the barrier to trophoblast attachment in the non-receptive uterus and that hormone-mediated changes in the epithelial plasmalemma are crucial to allowing adhesion and attachment at ovo-implantation. Clarifying the mechanisms for development of synchrony between the uterus and embryo is vital to understanding the implantation process and, as such, has relevance to fertility regulation in both humans and other animal species.