HORMONAL REGULATION OF MONKEY SERTOLI CELLS IN CULTURE

该项目的总体目标是评估生物化学和 睾丸和附睾对退缩或退缩的形态反应 剥夺FSH。抑制素治疗对第一波的影响 未成熟大鼠精子发生及其对精子发生和生育能力的影响 在老鼠和猴子身上都会进行调查。抑制素、雄激素结合水平 睾丸和附睾中的蛋白质(ABP)、睾酮和DHT将 在这些动物身上进行测量。促性腺激素、抑制素和 雄激素将被测量。抑制素在大鼠和牛体内的产生部位 牛睾丸和培养的支持细胞将被电子定位 显微免疫细胞化学技术。 FSH-亚基主动免疫对雄性食蟹的影响 将使用以下参数来调查猴子(M.Fasularis) 监测器：a)利用光和电子技术改变睾丸形态 显微技术，b)精液分析，包括精子计数、活动率、 结构、抑制素和雄激素水平，c)睾丸和 附睾和d)外周雄激素、黄体生成素和任何游离残存的卵泡刺激素水平。 抑制素水平将用放射免疫测定法和放射受体测定。 化验。免疫猕猴血浆中的外周抗体效价 将在进程中与上述参数相关联 免疫接种。 抑制素-卵泡刺激素-睾酮的相互关系将用抗性腺激素来探索 血清，以及在大鼠和猴子的脑下垂体切除。 这些研究对于更好地理解这一角色具有潜在的重要意义。 FSH在男性生殖中的作用。此外，关于抑制素的拟议研究还将， 为FSH分泌的调控提供新的有意义的信息。 这些方法有望为药理学提供有效的手段 封锁FSH用于基础研究和可能的应用。

The overall objective of this project is to evaluate the biochemical and morphological response of the testis and epididymis to a withdrawal or deprivation of FSH. The effects of treatment with inhibin on first wave of spermatogenesis in the immature rat as well as on spermatogenesis and fertility in the rat and monkey will be investigated. Levels of inhibin, Androgen Binding Protein (ABP), Testosterone, and DHT in the testis and epididymis will be measured in these animals. Circulating levels of gonadotropins, inhibin and androgens will be measured. Site of production of inhibin in rat and bovine Sertoli cells in culture and in bovine testis will be localized by electron microscopic immunocytochemical techniques. The effects of active immunization with FSH--subunit on the male crab-eating monkey (M. fasicularis) will be investigated, using the following parameters to monitor: a) changes in testicular morphology using light and electron microscopic techniques, b) semen analysis, including sperm count, motility, structure, levels of inhibin, and androgens, c) ABP levels in the testis and epididymis and d) peripheral levels of androgens, LH and any free residual FSH. The inhibin levels will be measured using radioimmunoassay and radioreceptor assay. The peripheral antibody titres in the plasma of the immunized monkeys will be correlated with the above parameters during the progress of immunization. Inhibin-FSH-Testosterone interrelations will be explored using antigonadotropic sera, and hypophysectomy in the rat and the monkey. These studies are of potential importance to a better understanding of the role of FSH in male reproduction. The proposed studies in inhibin will, in addition, provide new and significant information on the modulation of FSH secretion. These approaches hold the promise of providing effective means for pharmacologic blockade of FSH for both basic research and possible applications.