EXTREMELY RAPID GONADOTROPIN ASSAYS

人口控制和疾病诊断与治疗的问题 不孕不育，对当今社会具有重大意义。集成到 负责这些物质的是脑下垂体和性腺的分析系统。 控制生殖功能的荷尔蒙。目前，这种检测方法 通常需要2到4天的时间来执行。最近，通过优化 传统的人体竞争结合免疫分析技术 促黄体生成素(HLH)，我们已经开发出一种只需要2 几个小时的表演。这项检测目前正在本院常规使用。 我们现在建议开发所谓的“双部位”非竞争性分析方法，用于 人促黄体生成素、卵泡刺激素和绒毛膜促性腺激素 可以在15分钟内完成。这样的化验将使用高度精选的 用125I标记的单抗(或更晚标记的酶)作为 第一试剂。这种试剂被大量使用，允许快速使用 反应。第二个多克隆抗体针对多个其他 激素上的决定因素被用来分离标记的“结合”的单抗 来自“自由”的抗体。这样的“短期回转”化验将允许 在首次就诊期间对患者进行评估，以及 排卵峰发生时的测定。这样的方法 将在产科/妇科中心广泛适用和使用 和内分泌中心治疗不孕症，并用于控制 生育能力。

Problems of both population control, and diagnosis and treatment of infertility, are of major importance to today's society. Integral to caring for such matters are assay systems for pituitary and gonadal hormones that control reproductive functions. Currently, such assays usually require 2 to 4 days to perform. Recently, by optimizing conventional competitive binding immunoassay techniques for human luteinizing hormone (hLH), we have developed an assay that requires only 2 hours to perform. This assay is currently in routine use in our hospital. We now propose to develop so-called "two-site" noncompetitive assays for human LH, follicle stimulating hormone, and chorionic gonadotropins that can be performed in 15 minutes. Such assays would utilize highly selected monoclonal antibodies labeled with 125I (or later enzyme labeled) as a first reagent. This reagent is used in "great excess," permitting rapid reaction. A second polyclonal antibody directed against multiple other determinants on the hormone is used to separate labeled "bound" monoclonal antibody from "free." Such "short turn-around" assays will permit evaluation of a patient during the initial clinic visit, as well as determination of the ovulatory surge during its occurrence. Such methods would be widely applicable and usable in Obstetrical/Gynecological centers and Endocrine centers treating infertility, and for the control of fertility.