Quantifying reactive species in biological systems

该项目旨在发现活性氧和氮物种如何影响细胞功能、氧化应激和细胞功能障碍。潜在的机制通常是未知的，因为测量活性物种和相关氧化应激的方法不够充分。该项目将合成多用途探头，并将其与基于质谱学的分析和数学建模相结合。这将允许以一种与时间相关的方式同时量化特定细胞隔间中的活性物种，以反映它们在复杂生物系统中复杂的相互作用和代谢。该项目可以阐明活性物质是否以及如何影响细胞功能、衰老和疾病。

This project aims to discover how reactive oxygen and nitrogen species affect cell function, oxidative stress and cell dysfunction. The underlying mechanisms are often unknown because methods to measure reactive species and associated oxidative stress are inadequate. This project will synthesise multi-purpose probes and combine these with mass spectrometry-based analytics and mathematical modelling. This will allow concurrent quantification of reactive species in specific cellular compartments in a time-dependent manner that reflects their intricate interaction and metabolism in complex biological systems. This project could clarify whether and how reactive species affect cell function, ageing and disease.