FRAMEWORK LINKAGE MAPS FOR CHROMOSOMES 2 6 7 8 12 & 14

我们建议构建人类遗传连锁图谱的框架 染色体2、6、7、8、12和14。 地图将包括以下标记： 最小杂合性为70%的DNA序列多态性和标记 一般来说，间隔不超过10%的重组。 略高 如果标记信息量充足，则允许重组间隔 远高于70%。 初始图谱（男性、女性和性别 平均值）将从CEPH参考家系基因型构建， 连锁分析程序包CRI-MAP，这些将作为 确定需要探针开发的区域的基础。 CA重复 多态性将是待鉴定的新标记的主要类型， 表征了半自动图像分析程序将被开发为 有助于CA多态性的基因型分析。 起始组 提出来自每个染色体的候选索引标记用于研究。 一 测试双等位基因标记可行性的新方法 自动化基因分型测定被提议用于14号染色体定位， 与更传统的方法相结合。

We propose to construct framework genetic linkage maps for human chromosomes 2, 6, 7, 8, 12, and 14. The maps will consist of markers for DNA sequence polymorphism with minimum heterozygosities of 70% and marker spacing, in general, of no more than 1 0% recombination. Slightly higher recombination intervals will be allowed provided marker informativeness is considerably higher than 70%. Initial maps (male, female, and sex average) will be constructed from CEPH reference pedigree genotypes using the linkage analysis program package CRI-MAP and these will serve as the basis for determining regions requiring probe development. CA repeat polymorphisms will be the main type of new markers to be identified and characterized. Semiautomated image analysis programs will be developed as an aid to genotype analysis for CA polymorphisms. Initial sets of candidate index markers from each chromosome are proposed for study. A novel approach to test the feasibility of bi-allelic markers and automated genotyping assays is proposed for chromosome 14 mapping in conjunction with more traditional approaches.