CYCLIC NUCLEOTIDE METABOLISM IN LUNG & VASCULAR TISSUES

环核苷酸在肺和某些组织中的形成和作用的调节 包括气管平滑肌在内的细胞类型。 的 环磷酸鸟苷和环磷酸腺苷在气管平滑肌中的积聚 含有各种激素、神经激素、亚硝基化合物和其他物质 将与肌肉运动和内源性蛋白质相关 磷酸化 外源性和内源性蛋白质底物的循环 肺和气管平滑肌中的核苷酸依赖性蛋白激酶 将被审查。 将表征肺中的鸟苷酸环化酶， 纯化并用于获得抗体，所述抗体将用于开发 放射免疫测定法和免疫组织化学定位的酶， 酶在肺 因此，实验将用肺和一些 它的细胞类型，以确定哪些代理调节积累， 环核苷酸的作用，试图将某些功能归因于 环磷酸腺苷和环磷酸鸟苷。

The regulation of cyclic nucleotide formation and action in lung and some of its cell types including tracheal smooth muscle will be examined. The accumulation of cyclic GMP and cyclic AMP in tracheal smooth muscle induced with various hormones, neurohormones, nitroso compounds and other materials will be correlated with muscle motility and endogenous protein phosphorylation. Exogenous and endogenous protein substrates for cyclic nucleotide dependent protein kinases in lung and tracheal smooth muscle will be examined. Guanylate cyclase in lung will be characterized, purified and used to obtain antibodies that will be used to develop a radioimmunoassay for the enzyme and immunohistochemical localization of the enzyme in lung. Thus, experiments will be performed with lung and some of its cell types to determine which agents regulate the accumulation and action of cyclic nucleotides in an attempt to ascribe some functions to cyclic AMP and cyclic GMP in lung.