CULTURE AND CHARACTERIZATION OF THE ADULT HEART MYOCYTE

这项研究的长期目标是进一步表征 成年大鼠心室肌细胞的长期培养 并确定它是否恢复了分裂的能力。 具体的目标是 确定这些细胞是否具有类似于 在体内，成年心肌细胞恢复为更新生细胞类型， 这两种细胞类型的组合或具有 这是成人培养的肌细胞所特有的。 主要目的是确定如何 与体内心室心肌相比， cell. 待研究的参数包括其代谢模式， 肌球蛋白合成和亚型，Ca++激活的肌球蛋白ATP酶活性， 肌酸激酶活性及同工酶谱。 评估是否 这些细胞重新获得分裂的能力，它们将在 倒置的光学显微镜，以便可以观察单个细胞 一段时间 他们的行为将被一段延时录像记录下来 录音机。 进一步的研究将关注如何确定DNA 复制和细胞分裂在心室肌细胞中受到调节 使用最近分离的人胸苷激酶基因作为探针。 成年人心肌细胞体外培养的建立及其特性 培养将为研究分子生物学提供有价值的系统， 成体心脏细胞的生物化学、生理学和药理学。 如果我们 能够理解控制分化的机制， 成人心肌细胞的增殖，那么它可能是可能的， 可用于启动或加快维修的设计程序， 成年心肌损伤后的再生， 心肌梗塞

The long-term objective of this research is to further characterize the adult rat ventricular cardiac muscle cell maintained in long term culture and to determine if it regains the ability to divide. Specific aims are to determine whether these cells have characteristics which are similar to the in vivo adult heart myocyte, revert back to a more neonatal cell type, are a combination of both of these cell types or have characteristics which are unique to the adult cultured myocyte. The main aim is to determine how different they are compared to the in vivo ventricular cardiac muscle cell. Parameters to be investigated include their pattern of metabolism, myosin synthesis and isoform type, Ca++ - activated myosin ATPase activity, and activity and isoenzyme pattern of creatine kinase. To assess whether these cells regain the ability to divide they will be grown on the stage of an inverted optics microscope so that individual cells can be observed over a period of days. Their behavior will be recorded using a time-lapse video recorder. Further studies will be concerned with determining how DNA replication and cell division are regulated in the ventricular myocyte using as a probe a recently isolated human thymidine kinase gene. Establishment and characterization of adult cardiac myocytes in long-term culture will provide a valuable system to study the molecular biology, biochemistry, physiology and pharmacology of the adult heart cell. If we can understand the mechanisms which control the differentiation and proliferation of the adult heart muscle cell, then it may be possible to design procedures which can be used to initiate or expedite repair or regeneration of the adult myocardium following injury such as that caused by a myocardial infarction.