CAPILLARY AND INTERSTITIAL FLUID AND SOLUTE EXCHANGE

在许多病理条件下，存在着一个不平衡的分配， 血管和血管外空间之间的液体和溶质。 变化 在间隙凝胶基质的水合作用中， 血浆蛋白在这两个区室之间的分布， 流体分布。 拟议研究的总体目标是 确定血浆蛋白分布和转运的变化， 皮肤和骨骼肌的肌张力，并将数据与 介绍有关控制体内液体平衡的概念。 我以前的 研究表明，血浆蛋白的血管外分布， 皮肤和骨骼肌在盐水扩张或血浆置换后， 与静脉压升高或 毛细血管壁通透性 这可能会产生重要的后果， 血管和间质空间之间的液体平衡，因为主要 总身体间质液的一部分由皮肤表示， 骨骼肌 我建议检验血浆蛋白质是 直接从血浆通过体内的自由液体通道进入淋巴 静脉充血或内皮细胞改变后的血栓 渗透性和通过间隙凝胶基质， 血浆置换法 我将在皮肤和骨骼肌中研究这一点， 麻醉家兔的后腿。 我会比较平衡率 淋巴液中的示踪白蛋白，以确定 白蛋白是通过自由液体通道还是通过 凝胶基质 我还将确定不同形式的水肿的影响 白蛋白的静电排斥 这将通过研究 示踪白蛋白的血管外转运和分布， 改变收费但不改变大小。 更多的研究将比较 血管外高蛋白血症与低蛋白血症 皮肤和骨骼肌中液体和血浆蛋白的分布。 这些结果将提供信息的作用间质 基质在组织液平衡中的作用，并提供对病因的深入了解 水肿的形成。

In many pathological conditions, there is an imbalance in the partition of fluid and solutes between the vascular and extravascular spaces. Changes in the hydration of the interstitial gel matrix will effect the distribution of plasma proteins between these two compartments and alter the fluid distribution. The general aim of the proposed study is to determine changes in the distribution and transport of plasma proteins in the interstitium of skin and skeletal muscle and relate the data to the present concepts on the control of fluid balance in the body. My previous studies indicate that the extravascular distribution of plasma proteins in skin and skeletal muscle following saline expansion or plasmapheresis is different from that following increased venous pressure or increased capillary wall permeability. This may have important consequences on the fluid balance between the vascular and interstitial spaces since the major fraction of the total body interstitial fluid is represented by skin and skeletal muscle. I propose to test the hypothesis that plasma proteins are carried directly from plasma to lymph through free fluid channels within the interstitium following venous congestion or altered endothelial permeability and through the interstitial gel matrix following plasmapheresis. I will study this in both skin and skeletal muscle in the hind leg of anesthetized rabbits. I will compare the equilibration rate for tracer albumin in the interstitium to that in lymph to determine whether albumin is transported through free fluid channels or through the gel matrix. I will also determine the effects of different forms of edema on electrostatic exclusion of albumin. This will be done by studying the extravascular transport and distribution of tracer albumin, which has an altered charge but not size. Additional studies will compare the effects of hyperproteinemia to those of hypoproteinemia on the extravascular distribution of fluid and plasma proteins in skin and skeletal muscle. These results will provide information on the role of the interstitial matrix in tissue fluid balance as well as provide insight into the etiology of edema formation.