NEUROCHEMICAL STUDIES OF CNS CARDIOVASCULAR CENTERS

目前有几种药物用于治疗高血压， 作用于中枢神经系统的心血管控制中心 系统 这类药物包括可乐定和α-甲基多巴， 是α 2肾上腺素能受体激动剂。 其他CNS受体也可以是 参与高血压状态，他们的身份可能会导致 开发新的抗高血压药物。 我们希望研究 五种神经递质及其相应受体在脑内的分布 中枢神经系统心血管控制中心（例如， 疑核、第X神经核、孤束核 和脊髓的中间外侧柱），以确定 哪些药物可能在这些部位起作用。 肾上腺素能标记物， 胆碱能、γ-氨基丁酸、谷氨酸和5-羟色胺传递 通过光镜组织化学检测其受体， 放射自显影 心脏和血管控制的受体浓度 成年大鼠的中枢也将在传入神经阻滞后确定， 轴突切断术 观察到的变化将表明， 位于突触前或突触后。 此外，浓度 心血管控制中心的神经递质和药物受体 新生儿和成年正常血压和自发性高血压大鼠将 被比较。 这种比较可能揭示了中枢神经系统的化学性质。 高血压潜在的递质系统故障

There are several drugs currently used to treat hypertension which appear to act on the cardiovascular control centers of the central nervous system. Such drugs include clonidine and Alpha-methyldopa, both of which are Alpha2-adrenergic receptor agonists. Other CNS receptors may also be involved in hypertensive states and their identity might lead to the development of new anti-hypertensive drugs. We wish to study the distribution of five neurotransmitter and their corresponding receptors in central nervous system cardiovascular control centers (for example, the nucleus ambiguus, nucleus of the Xth nerve, nucleus of the solitary tract and the intermediolateral column of the spinal cord), in order to determine which drugs may potentially act at these sites. Markers for adrenergic, cholinergic, gamma-aminobutyric acid, glutamate, and serotonin transmission and their receptors will be detected by light microscope histochemistry and autoradiography. Receptor concentrations of cardiac and vasomotor control centers in adult rats will also be determined after deafferentation and axotomy. The changes observed will indicate whether the receptors tested are localized pre- or postsynaptically. In addition, the concentration of neurotransmitter and drug receptors in the cardiovascular control centers of neonatal and adult normotensive and spontaneously hypertensive rats will be compared. This comparison might reveal the chemical nature of central transmitter system malfunction underlying hypertension.