LIPID INVOLVEMENT IN OXYGEN TOXICITY

有毒的氧物质与肺损伤有关。 证据表明 氧自由基和其他氧物种的参与是推论，但 基于目前对复杂生物学中氧化学的理解， 系统. 重要的是要了解有毒的氧物质是如何导致 组织损伤以及它们通过何种化学机制起作用。 总的来说，我们将研究脂质过氧化作用在急性 由于暴露于有毒氧气 物种可能已经形成。 由于尚未证明， 脂质过氧化作用是一个必要的步骤， 肺损伤，我们建议确定脂质过氧化是否是一个必不可少的 导致肺损伤或仅导致平行化学过程的事件。 两 模型系统将被检查。 第一种是肺损伤， 高氧中毒 第二个是佛波醇引起肺损伤的研究 肉豆蔻酸乙酸酯 我们将寻找过氧化产物的形成 可以直接追溯到已知的化学反应。 我们将尝试 以确定哪些化学物质负责引发 过氧化作用 在本研究中，我们将使用气相色谱-质谱联用技术 光谱技术，使我们能够定量分钟（皮克） 材料的数量。

Toxic oxygen species are implicated in causing lung injury. Evidence that oxy-radicals and other oxygen species are involved is inferential, but based on current understanding of oxygen chemistry in complex biological systems. It is important to understand how toxic oxygen species can cause tissue injury and by what chemical mechanisms they act. In general, we will investigate the role of lipid peroxidation in acute lung injury that results from exposure to conditions in which toxic oxygen species are presumably formed. Since it has not been demonstrated that lipid peroxidation is a necessary step in the mechanisms responsible for lung injury, we propose to determine if lipid peroxidation is an essential event leading to lung damage or only a parallel chemical process. Two model systems will be examined. The first involves lung damage resulting from hyperoxia. The second is a study of lung damage caused by phorbol myristate acetate. We will look for the formation of peroxidation products that can be directly traced to known chemical reactions. We will attempt to determine what chemical species are responsible for the initiation of peroxidation. In this study, we will use gas chromatography-mass spectrometric techniques that will allow us to quantitate minute (picogram) amounts of material.