PHYSIOLOGY AND PHARMACOLOGY OF CARDIOVASCULAR NEURONS

这项研究启动了对第一突触传递的研究 压力感受器反射。突触位于大脑的一个区域 延髓由内侧细胞、背内侧细胞和延髓细胞组成 孤束背外侧核。这一区域接收传入信号 从压力感受器通路以及许多其他中枢神经 系统区域，是心血管系统整合的主要场所。焦点 这个项目的特征将是电气和 这一区域神经元的药理学特性。在这些研究中， 这些细胞是用酶法分离的。我们已经开发了这种制剂 这第一次提供了研究这些性质的机会 参与心血管调节的单个细胞。膜片钳 将使用全细胞记录技术来确定离子 负责膜电位的电流，并检查 对假定的递质物质和可乐定等药物的反应 以及钙拮抗剂。这将提供有关以下内容的信息 哪些物质参与调节神经活动？ 使用了机械装置。这些单元格还将使用 免疫细胞化学技术。中的单元格的属性 血压正常的大鼠(WKY品系)将与自发性大鼠进行比较 高血压大鼠(SHR)。这些研究将作为未来工作的基础 关于压力感受器信息处理的研究 二级神经元。

This study initiates an investigation of transmission at the first synapse of the baroreceptor reflex. The synapse is located in an area of the medulla which is composed of cells of the medial, dorso-medial and dorso-lateral nuclei of the solitary tract. This area receives afferents from the baroreceptor pathways as well as from many other central nervous system areas and is a major site of cardiovascular integration. The focus of this project will be a characterization of the electrical and pharmacological properties of the neurons in this area. In these studies, the cells are isolated enzymatically. We have developed this preparation which provides, for the first time, the opportunity to study the properties of individual cells involved in cardiovascular regulation. The patch clamp technique for whole cell recording will be used to determine the ionic currents responsible for the membrane potentials and to examine the responses to putative transmitter substances and to such drugs as clonidine and the calcium antagonists. This will provide information concerning which substances are involved in regulating the neural activity and what mechanisms are used. The cells will also be characterized using immunocytochemical techniques. The properties of the cells from normotensive rats (WKY strain) will be compared with those from spontaneous hypertensive rats (SHR). The studies will serve as a foundation for future studies examining the processing of baroreceptor information by these second order neurons.