PHYSIOLOGY AND PHARMACOLOGY OF CARDIOVASCULAR NEURONS

这些研究的长期目标是解决 心血管系统的神经调节，特别是心率 和动脉压。 电生理学和 解剖学技术提供了检查 参与调节的反射回路的中枢神经成分 神经流出到心脏和血管。 这里提出的工作是 一项电生理和免疫细胞化学研究， 内侧动脉压力感受器和其他外周和 中央来源。 第一个目的是检查的生理作用， 在孤立的mNTS中识别和表征的六种电流 神经元 这些电流是神经元自发活动的基础， 确定细胞将动作电位传递到细胞的能力。 压力感受器反射通路中的下一个细胞。 本部分研究将 利用传统的微电极电压记录和特定的 通道阻滞剂。 调制这一领域，然后将研究作为太 会影响压力反射通路的活动 两个这样 物质，谷氨酸和GABA，在同一个电话中被释放， 突触终扣与神经元共同分离，作为神经元的残余。 传入神经支配 这是第二个目标的基础， 继续研究兴奋性和抑制性突触机制 负责自发和刺激的递质释放。 初步证据表明，突触前调制这种释放 也将被追究。 第三个目标将探讨 其他潜在的递质物质，包括 儿茶酚胺、血清素和P物质。第四个目标是 与其他研究同时进行。 这些神经元 其特征在于电和发射器响应也将 使用免疫细胞化学和荧光技术在 光和电子显微镜水平的电位存在 索马和附着的终扣中的传递物质。 结果 将提供通过压力感受器的传输信息 反射和什么调节它，以及提供基本信息， 中枢突触传递的机制。

The long term goal of these studies addresses the mechanisms of the neural regulation of the cardiovascular system, in particular heart rate and arterial pressure. Recent developments in electrophysiological and anatomical techniques have provided the opportunity to examine the central neural components of the reflect loops involved in regulating neural outflow to the heart and blood vessels. The work proposed here is an electrophysiological and immunocytochemical study focusing on the cells of the medial arterial baroreceptors and other peripheral and central sources. The first aim is to examine the physiological role of six currents that have been identified and characterized in isolated mNTS neurons. These currents underlie spontaneous activity in the neuron and determine the ability of the cells to transmit action potentials to the next cells in the baroreflex pathway. This portion of the study will utilized conventional microelectrode voltage recording and specific channel blockers. Modulation of this area will then be studied as too will influence the activity of the baroreflex pathway. Two of these substances, glutamate and GABA, are released on the same call from synaptic boutons that are co-isolated with the neurons as remnants of the afferent innervation. This is the basis of the second aim which continues a study of the excitatory and inhibitory synaptic mechanisms responsible for spontaneous and stimulated transmitter release. Preliminary evidence suggesting presynaptic modulation of this release will also be pursued. The third aim will explore the response of the neurons to other potential transmitter substance including the catecholamines, serotonin and substance P. The fourth aim will be pursued concurrent with the other studies. The neurons that are characterized by electrical and transmitter responses will also be examined using immunocytochemical and fluorescence techniques at the light and electron microscopic level for the presence of potential transmitter substances in the soma and attached boutons. The results will provide information on the transmission across the baroreceptor reflex and what modulates it as well as providing basic information about the mechanism of central synaptic transmission.