Beating Hearts at High Resolution: Adaptive High Resolution Selective Plane Illumination Microscopy

在这个项目中，我们将通过利用光学显微镜，高速成像和自适应光学的发展来拍摄生命在心脏中的发展。这些心脏将在斑马鱼和老鼠的胚胎中存活。这是非常具有挑战性的，因为活的心脏很大（在微观尺度上），嵌入动物体内（这意味着它必须在深度成像），并且正在跳动，因此以每分钟约300次的速度移动。除了为证明我们将开发的先进方法提供挑战外，心脏发育的研究对于调查健康心脏的发育和确定心脏问题的原因都很重要。

In this project we will film life as it develops in the heart by exploiting developments in optical microscopy, high speed imaging, and adaptive optics. The hearts will be in live, developing zebrafish and mouse embryos. This is extremely challenging as the living heart is large (on a microscopic scale), is embedded in the body of the animal (meaning it must be imaged at depth) and is beating, and therefore moving, at a rate of around 300 beats per minute. As well as providing a challenge to demonstrate the advanced methods we will develop the study of cardiac development is important both for investigating the development of the healthy heart and for determining the causes of heart problems.