NEUROTRANSMITTERS AND LOCAL CIRCUITS IN SOMESTHESIS

拟议的研究的重点是识别 脊神经节神经元亚群中的神经递质，其 中央表示，尤指在丘脑楔状核的水平上 猫，以及这个核团的内部电路。 这项研究的目的之一是识别背神经节神经元 用谷氨酸作为神经递质，确定它们的百分比和 是否主要与皮肤、肌肉或关节有关 传入器。组织化学、放射自显影和免疫细胞化学 在大多数实验中，将使用技术，并结合使用更多 将采用不止一种方法来对神经元进行双重标记。 研究的第二个目的是研究乔木化的模式， 侧化、终止和个体的神经递质， 电生理识别的初级传入神经从前肢进入 猫的楔状核。对于这个一般的项目也不同 将采用实验策略，但重点是 细胞内记录和辣根过氧化物酶染色。 这项研究的第三个也是最后一个目标是从调查中确定 对含有谷氨酸脱羧酶的过程，因此， 推测使用GABA作为抑制性神经递质，内在的 目的：阐明丘脑楔状核的环路及其解剖基础。 其中的综合机制。 这项研究的总体目标是促进对 哺乳动物躯体感觉系统的基本功能特性 这是特别值得的，因为考虑到大部分 一些合成的研究是在丘脑和大脑的水平上进行的 大脑皮层。这种研究的重要性程度是必然的。 将会受到对运行在 丘脑前水平，即在背侧柱核和脊髓。

The proposed research's focus is on the issue of identification of neurotransmitters in a subpopulation of spinal genglia neurons, their central representation, especially at the level of the cuneate nucleus of cats, and the intrinsic circuitry of this nucleus. One of the aims of the research is to identify dorsal ganglion neurons that use glutamate as neurotransmitter, to establish their percentage and whether these are primarily related to cutaneous, muscle or joint afferents. Histochemical, autoradiographic and immunocytochemical techniques will be used and, in most experiments, a combination of more than one approach will be employed in order to doublelabel neurons. The second aim of the research is to study the pattern of arborization, collateralization, termination and the neurotransmitter of individual, electrophysiologically-identified primary afferents from the forelimb into the cuneate nucleus of cats. Also for this general project different experimentl strategies will be employed but the emphasis is on intracellular recording and staining with horseradish peroxidase. The third and final aim of the research is to define, from an investigation on processes that contain glutamic acid decarboxylase and are, therefore, presumed to use GABA as inhibitory neurotransmitter, the intrinsic circuitry of the cuneate n. and to elucidate the anatomical substrate of integrative mechanisms in it. The general goal of the research is to contribute to an understanding of basic functional properties of the somatosensory system in mammals a task that is made especially worth when considering that the bulk of the research on somesthesis is at the level of the thalamus and cerebral cortex. The degree of significance of such a research is by necessity going to be conditioned by the understanding of the mechanisms operating at a pre-thalamic level, i.e. in dorsal column nuclei and spinal cord.