CAPILLARY ELECTROPHORESIS ANALYSIS OF SINGLE NEURONS

描述(改编自申请人的摘要)：了解如何 中枢神经系统疾病总体上可以扰乱功能，这势在必行 来研究神经元之间化学连接的本质。 也许人类大脑中的一万亿个神经元共同发挥作用 通过10(15)个突触，以及许多神经元假设的概率 独特的身份要求进行敏感的化学分析 不同细胞间的差异。分子组成的测定 在细胞和亚细胞水平上对发展 神经细胞正常运行机制的精确模型 以及这些机制在疾病状态下如何变得有缺陷。 这项研究计划的长期目标是开发毛细管 高灵敏度荧光检测的电泳法 单个神经元、静脉曲张、突触、亚细胞的化学分析 隔间和释放处。目前，现场仪表是 能够分离和检测低至1700个分析物分子 取自大规模样品制备。对分钟实数的分析 然而，世界样本对样本施加了严格的限制 操纵。为了便于进行定量的微尺度分析， 荧光标记程序必须适应于柱上标记 天然的非荧光分析物。

DESCRIPTION (Adapted from applicant's abstract):To understand how diseases of the CNS can disrupt functions on the whole, it is imperative to investigate the nature of chemical connectivity between neurons. Perhaps one trillion neurons within the human brain function together through 10(15) synapses, and the probability that many neurons assume unique identities demands chemical analyses that are sensitive to variations from cell to cell. Determination of the molecular composition at the cellular and sub-cellular level is critical to development of an accurate model of the mechanisms by which nerve cells normally communicate and how these mechanisms can become flawed in disease states. The long-term objective of this research program is to exploit capillary electrophoresis with high sensitivity fluorescence detection to perform chemical analyses of single neurons, varicosities, synapses, subcellular compartments, and releasates. Presently, on-site instrumentation is capable of fractionating and detecting as few as 1700 analyte molecules taken from a large scale sample preparation. Analysis of minute real- world samples, however, places stringent limitations on sample manipulations. To facilitate quantitative microscale analyses, fluorescent-tagging procedures must be adapted for on-column labeling of natively non-fluorescent analytes.