NEUROTRANSMITTERS AND LOCAL CIRCUITS IN SOMESTHESIS

建议的研究重点是识别问题， 脊髓神经节神经元亚群中的神经递质，它们的 中央代表，特别是在楔状核的水平 猫和这个细胞核的内在电路 这项研究的目的之一是识别背神经节神经元， 使用谷氨酸作为神经递质，以确定其百分比， 无论这些疾病是否主要与皮肤、肌肉或关节相关 传入神经 组织化学、放射自显影和免疫细胞化学 技术将被使用，在大多数实验中， 将采用不止一种方法来使神经元加倍。 本研究的第二个目的是研究树枝化模式， 侧支化，终止和个体的神经递质， 电生理学鉴定的初级传入从前肢进入 猫的阴部神经核 也为这个一般的项目不同 将采用实验策略，但重点是 细胞内记录和辣根过氧化物酶染色。 研究的第三个也是最后一个目的是通过调查确定 在含有谷氨酸脱羧酶的过程中，因此， 推测使用GABA作为抑制性神经递质，内在的 电路的电流。并阐明 整合机制在其中。 研究的总体目标是帮助理解 哺乳动物躯体感觉系统的基本功能特性 这是特别值得的，当考虑到大部分的 对某些感觉的研究是在丘脑和大脑水平上进行的 皮层 这种研究的重要程度是必要的 将取决于对运行机制的理解 丘脑前水平，即在背柱核和脊髓中。

The proposed research's focus is on the issue of identification of neurotransmitters in a subpopulation of spinal genglia neurons, their central representation, especially at the level of the cuneate nucleus of cats, and the intrinsic circuitry of this nucleus. One of the aims of the research is to identify dorsal ganglion neurons that use glutamate as neurotransmitter, to establish their percentage and whether these are primarily related to cutaneous, muscle or joint afferents. Histochemical, autoradiographic and immunocytochemical techniques will be used and, in most experiments, a combination of more than one approach will be employed in order to doublelabel neurons. The second aim of the research is to study the pattern of arborization, collateralization, termination and the neurotransmitter of individual, electrophysiologically-identified primary afferents from the forelimb into the cuneate nucleus of cats. Also for this general project different experimentl strategies will be employed but the emphasis is on intracellular recording and staining with horseradish peroxidase. The third and final aim of the research is to define, from an investigation on processes that contain glutamic acid decarboxylase and are, therefore, presumed to use GABA as inhibitory neurotransmitter, the intrinsic circuitry of the cuneate n. and to elucidate the anatomical substrate of integrative mechanisms in it. The general goal of the research is to contribute to an understanding of basic functional properties of the somatosensory system in mammals a task that is made especially worth when considering that the bulk of the research on somesthesis is at the level of the thalamus and cerebral cortex. The degree of significance of such a research is by necessity going to be conditioned by the understanding of the mechanisms operating at a pre-thalamic level, i.e. in dorsal column nuclei and spinal cord.