NEUROCHEMICAL CORRELATES OF CEREBELLAR DEVELOPMENT

乙酰胆碱，γ-氨基丁酸， 小脑皮质中的去甲肾上腺素、血清素和组胺 将通过光学显微镜放射自显影观察小鼠， 受体结合配体。 受体定位于 和/或小脑皮质层在出生后的发展将是 研究特别强调在发展阶段， 首先出现。 我们将尝试将受体的出现 与其他已知的个体发生事件，如细胞迁移和突触 阵 基因突变影响了结构， 小脑皮质的组织和发育将被用于 旨在阐明神经递质细胞定位的研究 受体和/或其正常发育模式中的任何异常。 为了确定受体的出现和定位在多大程度上 由小脑皮层的有序结构和连接所控制， 来自大鼠小脑的细胞将在分散的细胞培养物中生长， 通过受体结合配体的放射自显影显现受体 不同的发展阶段。 小脑发育的阶段 培养受体成为“功能性”将由 2-脱氧-D-葡萄糖利用变化的放射自显影定位 在施用受体激动剂之后。 单克隆抗体， 针对大鼠小脑神经元的细胞表面， 小鼠以获得鉴定神经元细胞所需的试剂 文化中的类型。 这些神经化学研究可能会带来更好的 了解影响神经系统的故障。

Neurotransmitter receptors for acetylcholine, gamma-aminobutyric acid, noradrenaline, serotonin and histamine in the cerebellar cortex of the mouse will be visualized by light-microscopic autoradiography of receptor-bound ligands. The localization of the receptors to regions and/or layers of the cerebellar cortex during postnatal development will be studied with special emphasis on the developmental stage at which receptors first appear. We will attempt to correlate the appearance of receptors with other known ontogenetic events, such as cell migration and synapse formation. MIce with genetic mutations affecting the structure, organization and development of the crebellar cortex will be used in studies aimed at elucidating the cellular localization of neurotransmitter receptors and/or any abnormalities in their normal pattern of development. to determine to what extent receptor appearance and localization is governed by the orderly structure and connections of the cerebellar cortex, cells from the rat cerebellum will be grown in dispersed cell culture and receptors visualized by autoradiography of receptor-bound ligands at various developmental stages. The stage of cerebellar development in culture at which receptors become "functional" will be studied by the autoradiographic localization of changes in 2-deoxy-D-glucose utilization following administration of receptor agonists. Monoclonal antibodies, directed against cell surfaces of rat cerebellar neurons will be raised in mice to obtain reagents necessary for the identification of neuronal cell types in culture. These neurochemical studies could lead to a better understanding of malfunctions affecting the nervous system.