FLUORESCENT IMMUNO LIPOSOMES FOR PARTICLE TRACKING

单标记粒子的跟踪是研究单标记粒子的有力手段。 细胞表面的动力学。然而，只有少数几种粒子是 可用于跟踪和应用的这些粒子是有限的。 我们建议开发和表征灵敏的、特定的荧光 标记免疫脂质体用于粒子示踪和其他光学 技巧。我们将探索磷脂成分和荧光 给出最明亮的脂质体的标签和研究方法 将抗体结合到这些脂质体上以产生低价颗粒。 免疫脂质体在细胞表面的行为将是 以修补、封顶和横向扩散为特征。 标签的开发应允许粒子跟踪的应用 以及激光镊子技术应用于各种类型的细胞，包括 圆形而厚实的单元格，其图像在目前并不容易使用 单粒子技术。

Tracking of single marker-particles is a powerful method for studying the dynamics of cell surfaces. However, only a few types of particles are available for tracking and applications of these particles are limited. We propose to develop and characterize sensitive, specific fluorescent marker immunoliposomes useful for particle tracking and other optical techniques. We will explore phospholipid compositions and fluorescent labels that give the brightest possible liposomes and study ways of conjugating antibodies to these liposomes to yield paucivalent particles. The behavior of the immunoliposomes on the cell surface will be characterized in terms of patching, capping and lateral diffusion. Development of the labels should allow application of particle tracking and laser tweezers techniques to a wide range of cell types, included rounded and thick cells whose images are not easily used in present single particle techniques.