GENETICS OF SUBSTITUTED AROMATIC COMPOUND METABOLISM

土壤细菌的分解代谢基因为研究提供了一个独特的系统 原核生物基因调控的基本过程。主 这项研究的目的是探索池中的分歧 编码取代芳香族化合物的结构基因 分解代谢。我们建议描述和澄清 酶在延长新陈代谢途径中具有战略意义 细菌宿主的表型能力。我们将研究 这些战略酶对红曲霉菌利用的贡献 化石、可再生和合成化合物。这些研究将 也使我们能够将这些分子的结构和功能联系起来 酵素。为此，基因的结构和调控 编码氯代儿茶酚双加氧酶和单加氧酶将是 分析过了。我们感兴趣的两种类型的单加氧酶是 水杨酸羟基酶和2，4-D单加氧酶。 另一个目标是研究调节细菌的基因 取代芳香族化合物氧化的分解代谢途径 化合物合成三元酸循环中间体的研究 互补、重组DNA等技术。监管 控制氯芳烃分解代谢的基因、TFDR/S和TFDT 2，4-二氯苯氧乙酸酯(2，4-D)和1，4-二氯苯的DcbR 降解，将在分子水平上进行剖析。角色 在由三个2，4-D调控基因编码的蛋白质中 检查过了。 第三个目标是研究丹参的作用机制。 单个和多个调控元件及其分子细节 2，4-D和2，4-D融合通路的部分表达 1，4-二氯苯分解代谢。

Catabolic genes of soil bacteria offer a unique system for studying fundamental processes of procaryotic gene regulation. The main objective of this research is to explore the divergence in a pool of structural genes encoding substituted aromatic compound catabolism. We propose to characterize and clarify the role of enzymes strategically located in a metabolic route in extending the phenotypic capabilities of bacterial hosts. We will examine the contribution of these strategic enzymes to the utilization of fossil, renewable, and synthetic compounds. These studies will also enable us to correlate the structure and function of these enzymes. To this end, the structure and regulation of genes encoding chlorocatechol dioxygenases and monooxygenases will be analyzed. Two types of monooxygenases of our interest are salicylate hydroxylase and 2,4-D monooxygenase. Another objective is to study bacterial genes that regulate catabolic pathways governing the oxidation of substituted aromatic compounds to tricarboxylic acid cycle intermediates using complementation, recombinant DNA and other techniques. Regulatory genes controlling chloroaromatic catabolism, tfdR/S and tfdT of 2,4-dichlorophenoxyacetate (2,4-D) and dcbR of 1,4-dichlorobenzene degradation, will be dissected at the molecular level. The role of the proteins encoded by three 2,4-D regulatory genes will be examined. The third objective is to investigate the action mechanisms of single and multiple regulatory elements and the molecular details of the partial expression of convergent pathways for 2,4-D and 1,4-dichlorobenzene catabolism.