DNA TOPOISOMERASES IN HEAT SHOCK GENE EXPRESSION

最近有几种药物被确定为特异性抑制剂 真核生物DNA拓扑异构酶。 我们已经证明， 抑制剂影响真核细胞热休克基因的调控 表达表明这些酶在这种高度表达中的作用。 保守的真核细胞功能。 本提案的重点是 就是用这些抑制剂来描述DNA的功能 拓扑异构酶在调节真核基因表达中的作用。 的 果蝇热休克系统将被用作这些的模型 问题研究

Several drugs have recently been identified as specific inhibitors of eukaryotic DNA topoisomerases. We have shown that these inhibitors affect the regulation of eukaryotic heat shock gene expression suggesting a role for these enzymes in this highly conserved eukaryotic cell function. The emphasis of this proposal is to use these inhibitors to delineate the function of DNA topoisomerases in regulating eukaryotic gene expression. The Drosophila heat-shock system will be used as a model for these studies.