MOLECULAR/CELLULAR ACTION OF CRANIN IN NEURAL CELLS

髓外基质蛋白层粘连蛋白在 刺激和引导轴突的生长。此外，它是 在发育和再生的大脑中平行表达 有活跃的轴突生长。层粘连蛋白的作用可能是 对促进脑损伤后的康复具有重要的临床意义， 因为含层粘连蛋白涂层表面的外源性移植物可以 允许轴突再生发生在中枢神经内 成年哺乳动物的系统。 神经细胞通过细胞表面分子与层粘连蛋白相互作用。这个 了解层粘连蛋白的轴突打印效果的第一步 可以被监管或便利的是了解身份和 层粘连蛋白结合蛋白在神经细胞中的作用 表面。这一提议检验了一种新的假设 已发现的层粘连蛋白结合蛋白Granin在 介导层粘连蛋白在神经细胞上的一些作用。 产生的抗体将阻断其与红蛋白的结合 层粘连蛋白。神经细胞将暴露在这些抗体中进行测试 他们对层粘连蛋白的行为反应是否会改变。 这些或其他抗生长素抗体也将被合金化 描述Tranin在发育和发育过程中的表达模式 再生大脑，并检查可能的结构 作为发育过程中的一种功能而发生的生长素的修饰 在不同的生理条件下，不同的组织类型。 例如，还将检查红蛋白与层粘连蛋白的结合 测试红蛋白是否与已知的层粘连蛋白结构域结合 轴突促进活性或其他生物活性。因此。一个大女人 直接和相关证据的主体将被引证到 暗示了脑红蛋白在神经细胞中所起作用的本质。 红蛋白的分子克隆将完成。这将允许 它的氨基酸序列和其他详细的结构特征 被演绎。此外，还将对红蛋白缺陷型细胞进行转基因 并测定层粘连蛋白的新反应性。 这将对卡林拟议的角色提供决定性的测试 一种层粘连蛋白“受体”。Cranin可能在调节 轴突在特定条件下伸展或再生的能力， 因此，重要的是要了解CRAIN的结构和 功能。通过克隆获得的信息可能会带来新闻手段 控制脑红蛋白的表达，从而促进 胎儿和成人的最佳轴突生长 在受到侮辱之后。

the extracullular matrix protein laminin is extremely potent in stimulating and guiding the growth of axons. Moreover, it is expressed in the developing and regenerating brain, in parallel with active axonal outgrowth. The effects of laminin may be clinically important in promoting recovery following brain insults, because exogenous grafts containing laminin coated surfacts can permit regeneration of axons to occur within the central nervous system of adult mammals. Neural cells interact with laminin via cell surface molecules. The first step in understanding how laminin's neurite printing effects can be regulated or facilitated is to learn the identities and roles of the laminin-binding proteins found on neural cell surfaces. This proposal tests the hypothesis that a newly discovered laminin-binding protein, cranin, is critical in mediating some of laminin's actions on neural cells. Antibodies will bs raised against cranin that block its binding to laminin. Neural cells will be exposed to these antibodies to test whether their behavioral responses to laminin will be altered. These or other anti-cranin antibodies will also be alloyed to describe the pattern of cranin's expression in developing and regenerating brain, and to examine possible structural modifications of cranin that occur as a function of developmental stags, types of tissue, or under different physiologic conditions. the binding of cranin to laminin will also be examined, for example to test whether cranin binds to a domain of laminin with known neurite promoting activity or other#r bioactivities. Thus. a broad body of both direct and correlative evidence will be adduced to suggest the nature of cranin's roles in neural cells. The molecular cloning of cranin will be completed. This will allow its amino acid sequence and other detailed structural features to be deduce. Furthermore, cranin deficient cells will be transfected with cranin cDNA and assayed for new responsiveness to laminin. This will provides a conclusive test of cranin's proposed role as a laminin 'receptor'. Cranin may be critical in regulating the ability of axons to extend or regenerate under certain conditions, hence it is important to understand cranin's structure and function. Information gained via cloning may lead to news means of controlling the expression of cranin in brain, and thus promote optimal axonal growth both in the fetus and in the adult human following insults.