NEONATAL NUTRITION AND RESONANCE IONIZATION SPECTROSCOPY

新生儿微量元素营养知识匮乏 要求，特别是对早产儿，其关键的最终结果 三个月时必需微量元素的积累突然停止 出生。这项建议的目标是发展科学和 技术“桥梁”需要证明直接、或 近直接共振电离质谱法测定 (Rims)小于或等于亚纳克量的钼、铜和钒 早产儿采血0.1 cm~(-3)。具体地说，目标是 (1)研究和应用共振电离方案 钼、铜、钒的元素选择性电离；(2)发展同位素 样品中微量化学物质的稀释分析方法 (3)论证了亚纳克级测量的可行性。 钼、铜和钒的量；(4)展示了集成和 上述组件的应用可以带来显著的进步 新生儿营养和这种新的超重分析的概念 该工具可扩展并广泛应用于元素和同位素分析。 较长期的(第二阶段)目标是：(1)设计和 构建具有成本效益的商业原型RIMS系统 结合了结构和使用的简单性和最大限度的元素 和同位素测量的灵活性；(2)演示了 从第一阶段到解决具体问题的知识和见解 新生儿营养。

A paucity of knowledge exists about neonatal trace element nutrition requirements, especially for premature infants, whose critical final trimester accumulation of essential trace elements is abruptly ceased at birth. The objective of this proposal is to develop the science and technology "bridge" required to demonstrate the feasibility of direct, or nearly direct, determination by resonance ionization mass spectrometry (RIMS) of sub-nanogram quantities of Mo, Cu, and V in less than or equal to 0.1 cm-3 blood samples from premature infants. Specifically, the aims are: (1) To investigate and apply resonance ionization schemes for the elementally selective ionization of Mo, Cu, and V; (2) To develop isotope dilution analysis methodology for samples with minimal chemical preparation; (3) Demonstrate the feasibility of measurement of sub-nanogram quantities of Mo, Cu, and V; (4) Demonstrate how the integration and application of the above components can lead to the significant advancement of neonatal nutrition and how the concept of this new ultratrace analytical tool can be expanded and applied broadly to elements and isotope analysis. The longer term (Phase II) objectives would be: (1) To design and construct a cost effective commercial prototype RIMS system that incorporates simplicity of construction and use and a maximum of elemental and isotopic measurement flexibility; (2) To demonstrate the application of knowledge and insight from Phase I to the solution of specific problems in neonatal nutrition.