NOVEL SUPPORTS FOR CARBOHYDRATE IMMOBILIZATION

生物活性分子在载体上的固定化 矩阵在生物技术中有着广泛的应用。被动性 生物分子(疏水和离子)对 聚合物矩阵是最不复杂的方法 固定化；然而，许多生物化学物质不能很好地吸收 塑料聚合物表面。特别是，碳水化合物通常是 很难用足够的坚韧来吸收。轻便， 碳水化合物部分的共价固定化 在植入性装置表面改性中的应用 使酶中碳水化合物抗原的检测成为可能 链接免疫吸附分析(ELISA法)程序。 我们建议使用Bio-Metric系统的专有技术来 展示了多糖光化学固定化 难处理的聚苯乙烯表面。预计这项技术将 这个项目的结果将导致 用于多个共价键的衍生载体材料 碳水化合物的程序比其他程序更不麻烦 共价链协议。

The immobilization of biologically active molecules on support matrices has many applications in biotechnology. Passive absorption of biomolecules (both hydrophobic and ionic) to polymeric matrices is the least complicated method of immobilization; however, many biochemicals do not absorb well to plastic polymer surfaces. In particular, carbohydrates are often difficult to absorb with a sufficient degrees of tenacity. Facile, covalent immobilization of carbohydrate moieties would have application in the surface-modification of implantable devices and make possible the detection of carbohydrate antigens in enzyme linked immunosorbent assay (ELISA) procedures. We propose to use Bio-Metric System's proprietary technologies to demonstrate photochemical immobilization of polysaccharides to intractable polystyrene surfaces. The technology expected to result from this project will lead to the development of derivatized support materials for covalent linkage of many carbohydrates by procedures which are less cumbersome than other covalent linkage protocols.