OXYGEN BIO-ANALYZER USING OPTICAL SENSOR TECHNIQUES

该项目的目标是开发和生产一种光学 体外对氧浓度变化敏感的光纤氧气微传感器 细胞培养物中的氧气浓度。这个传感器系统的价值 是在基于测量细胞培养、细胞中氧代谢的生物测试中 毒性研究、药物检测和环境质量检测。 第一阶段的目标是测试传感器设计的可行性 准确测定细胞氧气呼吸的速率和质量 在正常生长和各种毒性或刺激性条件下。 该传感器是通过将磷光指示剂化合物粘贴到 光纤的远端。激发光投射到 光纤激发磷光体发射波长移位的长寿命 衰变信号返回给探测器。荧光粉的发光受到影响 通过氧的存在来熄灭长寿命的发光。 因此，光学跟踪大气中的氧气水平是可行的。 传感器尖端细胞的微环境基于 磷光。 开发的技术将产生一系列多功能的生物检测产品 广泛应用于细胞代谢、毒理学、癌症等领域的研究 生物学、治疗学的发展和诊断学对临床医学的影响。

The project's objective is the development and production of an optical fiber-based oxygen microsensor which is sensitive in vitro to changes in oxygen concentration within cell cultures. The value of this sensor system is in bioassays based on measuring oxygen metabolism in cell cultures, cell toxicity studies, drug testing and environment quality testing. The aims of Phase I are to test the feasibility of the sensor design for accurately determining the rate and quality of cells' oxygen respiration during normal growth and under various toxic or stimulatory conditions. The sensor is fabricated by affixing a phosphorescent indicator compound to the distal tip of a fiber optic. Excitation light projected through the fiber excites the phosphor which emits a wavelength shifted long-lifetime decay signal returned to a detector. The phosphor's emission is affected by the presence of oxygen which quenches the long-lifetime luminescence. It is thereby practical to optically track oxygen levels in the microenvironment of cells at the sensor tip based on the lifetime of the phosphorescence. The technology developed will yield a family of versatile bioassay products with applications that span research in cell metabolism, toxicology, cancer biology, development of therapeutics, and diagnostics to clinical medicine.