RAPID CYTOMETRIC ASSAY FOR LYMPHOCYTE ACTIVATION

淋巴细胞活化的快速细胞计数检测具有许多潜力 临床应用，包括细胞免疫功能障碍的评价 在HIV感染和癌症中，评估自身免疫性 疾病过程、组织分型和移植受者监测 移植排斥反应的早期迹象 为了发展 可重复的，标准化的方法，可以在当前的 新一代的临床流式细胞仪，以及新一代的流式细胞仪 目前，多参数流式细胞仪已投入使用 分析并关联细胞质pH、荧光素 荧光偏振和激活抗原的表达 外周血T淋巴细胞在活化后的前24小时内 多克隆有丝分裂原植物血凝素或由同种异体抗原混合 淋巴细胞反应 很可能拟议的研究将产生 基于pH测量和活化抗原检测的可用测定。

Rapid cytometric assays for lymphocyte activation have many potential clinical applications, including evaluation of cellular immune dysfunction in HIV infection and cancer, assessment of the activity of autoimmune disease processes, tissue typing, and monitoring of transplant recipients for early signs of graft rejection. With a view toward the development of reproducible, standardized methods which can be implemented on the current generation of clinical flow cytometers, as well as on newer cytometric apparatus now coming into use, multiparameter flow cytometry will be used to analyze and correlate changes in cytoplasmic pH, fluorescein fluorescence polarization, and activation antigen expression of human peripheral T-lymphocytes during the first 24 hours following activation by the polyclonal mitogen phytohemagglutinin or by alloantigen in the mixed lymphocyte reaction. It is likely that the proposed studies will yield usable assays based on pH measurement and activation antigen detection.