ENZYMATIC SYNTHESIS OF SPECIFIC DIPEPTIDES FOR NUTRITION

描述：（改编自研究者摘要。）最近 研究表明，二肽可能具有几个优点， 游离氨基酸作为治疗性肠内营养的氮源， 肠外营养 如果要使用合成二肽， 彻底调查并最终纳入医疗实践， 需要有一种成本有效的方法来合成特定的 二肽 然而，可用的肽合成技术具有严重的缺陷。 缺点. 缺点包括在制备过程中组分的外消旋化。 偶联和脱保护反应，高成本和潜力 所用试剂造成的环境危害，以及昂贵和 每一步之后都需要耗时的纯化步骤。 该项目旨在证明简化的 和经济的肽合成方法。 该工艺基于 酶催化反应，克服了传统的缺点， 合成技术 第一阶段计划的目标有三个方面：1）证明 该系统可以合成和脱保护三个目标， 二肽，2）在制备条件下测试系统的性能， 规模，和3）进行初步评估的经济 提议的系统。 第一阶段的成功将导致第二阶段的建议 专注于将该技术扩展到更广泛的二肽， 对所提出的工艺进行了优化，并进行了详细的经济分析， 整个综合系统。

DESCRIPTION: (Adapted from the investigator's abstract.) Recent investigations indicate that dipeptides may have several advantages over free amino acids as the nitrogen source in therapeutic enteral and parenteral nutrition. If the use of synthetic dipeptides is to be investigated completely and ultimately incorporated into medical practice, it is necessary to have a cost-effective method for synthesizing specific dipeptides. However, available peptide-synthesis techniques have serious drawbacks. The drawbacks include racemization of components during coupling and deprotection reactions, the high cost and potential environmental hazards posed by the reagents used, and the expensive and time consuming purification steps required after each step. This project is directed at demonstrating the feasibility of a simplified and economical peptide-synthesis procedure. The process is based on enzyme-catalyzed reactions that overcome the disadvantages of conventional synthesis techniques. The objectives of this Phase I program are threefold: 1) to demonstrate that the proposed system can synthesize and deprotect three target dipeptides, 2) to test the performance of the system at a preparative scale, and 3) to conduct a preliminary assessment of the economics of the proposed system. Success in Phase I would lead to a Phase II proposal focused on expansion of the technology to a wider range of dipeptides, optimization of the proposed process, and a detailed economic analysis of the entire integrated system.