PHOTOCHEMICAL MODIFICATION OF HYBRIDIZATION MEMBRANES

描述(改编自申请人的摘要)：核转移 酸到膜过滤器提供了许多应用 分子杂交包括基因组结构分析和 传染病和遗传性疾病的功能和诊断。这个 杂交反应的敏感性和重复性取决于 所用膜的性能。BSI已经开发出一种专利 允许试剂共价偶联的光固定化技术 在众多的膜材料上。建议应用这项技术。 用于核酸杂交的膜的表面改性 化验。 第一阶段的目标是合成涂层试剂，以使 将光反应基团附着到膜表面， 能够与DNA共价键合；证明带正电荷 用来离子结合DNA的基团可以在DNA之后被移除 完成光固定化以减少背景问题；并定义 DNA转移、杂交和剥离探针的条件 改性膜。长期目标是开发一种能够 牢固地结合各种大小的DNA片段，以最低限度提供最大信号 背景，可多次重新探测，并可用于多个 格式在广泛的条件下。

DESCRIPTION (Adapted from applicant's abstract):The transfer of nucleic acids to membrane filters has provided a number of applications for molecular hybridization including the analysis of genome structure and function and diagnosis of infectious diseases and genetic disorders. The sensitivity and reproducibility of the hybridization reaction depend on the properties of the membrane used.BSI has developed a proprietary photoimmobilization technology that allows covalent coupling of reagents onto numerous membrane materials. It is proposed to apply this technology to modify the surface of membranes used in nucleic acid hybridization assays. The Phase I objectives are to synthesize coating reagents which will permit the attachment of photoreactive groups to membrane surfaces that are capable of covalently bonding to DNA; demonstrate that positively charged groups used to ionically bind the DNA can be removed after DNA photoimmobilization is complete to reduce background problems; and define conditions for DNA transfer, and hybridization and stripping of probes on modified membranes. The long-range goal is to develop a membrane that can firmly bind DNA fragments of all sizes, provide maximum signal with minimal background, can be reprobed multiple times, and can be used in a number of formats under a wide range of conditions.