IMMUNOASSAY FOR AVAROL

本项目的总体目标是开发免疫测定法 能够检测纳克水平的Avarol和Avarone， 生物样本 Avarol是一种海洋天然产品， 有效的抗肿瘤和抗艾滋病活性。 该化合物 作为抗艾滋病药物和抗肿瘤药物正在接受评估 美国国家癌症研究所的一名研究员。 迄今为止， 生物样品中Avarol的测定方法。 我们最近 成功地开发了用于小型非 免疫原性分子如克百威、涕灭威、沙冬青毒素 和河豚毒素，为我们生产阿伐洛尔做好了准备 免疫测定。 Avarol将化学选择性和区域选择性连接 免疫原性蛋白质通过两个不同的网站内 分子。 这将使我们能够揭露典型的 Avarol的不同表位。 通过使用这些获得的抗体 免疫原有望为临床化学家提供某种 检测阿伐洛尔存在的灵敏方法， 各种生物基质。 第一阶段还将侧重于 产生阿伐洛尔的初步免疫测定。 二期将 完善多克隆试验，并将集中于生产 这种化合物的单克隆抗体， 倍半萜对苯二酚。

The overall goal of this project is to develop immunoassays capable of detecting avarol and avarone at the nanogram level in biological samples. Avarol is a marine natural product with potent anti-tumor and anti-AIDS activity. This compound is undergoing evaluation as an anti-AIDS drug and an anti-tumor agent at the National Cancer Institute. To date there are no assay methods for avarol in biological samples. Our recent successes in developing linker technology for small non- immunogenic molecules such as carbofuran, aldicarb, palytoxin, and tetrodotoxin, have prepared us for the production of avarol immunoassays. Avarol will be chemo- and regioselectively linked to immunogenic proteins via two different sites within the molecule. This will enable us to expose characteristically different epitopes of avarol. Antibodies obtained by using these immunogens are expected to offer the clinical chemist a certain and sensitive method of detecting the presence of avarol in a variety of biological matrices. Phase I will also focus on generating a preliminary immunoassay for avarol. Phase II will refine the polyclonal assay and will concentrate on the production of monoclonal antibodies to this compound and closely related sesquiterpene hydroquinones.