METABOLIC IMAGING OF LIVERS AWAITING TRANSPLANT

这项研究的长期目标是开发无创光学 冷冻机功能完整性评估仪器和方法 移植前储存的肝脏。本研究是在工作的基础上进行的 假设在低温储存的肝脏中，功能失调的区域 氧化代谢可以无创地检测到 分光光度法。NADH和可见光的荧光检测 光反射检测细胞色素氧化还原的变化将用于 在常氧下检查低温灌流和冷藏的大鼠肝脏， 缺血型和非偶联型。L阶段研究将证明 工作假说的可行性。设备将组装成 成像肝脏NADH荧光并测量细胞色素反射光谱 从低温灌流和冷藏的大鼠肝脏中提取。的相关性 将根据肝功能和功能障碍进行光学改变。这个 将对开发的方法进行敏感性评估 根据背景区分线粒体代谢受损的区域 正常组织的组织。在成功演示工作后 假设，第二阶段将使这些概念适应成像的发展 方法并在肝移植的临床环境中进行测试 位于华盛顿州西雅图的哈博维尤医疗中心。

The long term goal of this research is to develop noninvasive optical instruments and methods for assessing the functional integrity of cold- stored livers prior to transplant. This research is based on the working hypothesis that in hypothermically-stored livers, areas of dysfunctional oxidative metabolism can be detected noninvasively using spectrophotometric methods. fluorescence detection of NADH and visible light reflectance detection of cytochrome redox changes will be used to examine hypothermic perfused and cold-stored rat livers under normoxic, ischemic and uncoupled conditions. The Phase l studies will demonstrate the feasibility of the working hypothesis. Equipment will be assembled to image hepatic NADH fluorescence and measure cytochrome reflectance spectra from both hypothermic perfused and cold-stored rat livers. Correlation of optical changes with hepatic function and dysfunction will be made. The methods developed will be evaluated for their sensitivity for distinguishing areas of impaired mitochondrial metabolism on a background of normal tissue. Upon successful demonstration of the working hypothesis, Phase II will adapt these concepts to development of imaging methods and to testing in a clinical environment at the liver transplant center at Harborview Medical Center, Seattle, Washington.