RAPID EIA FOR COTININE IN BIOLOGICAL FLUIDS

烟草产品的消费及其对 我们民众的总体健康，过去是，现在仍然是一个主要的健康 有问题。尽管消费香烟的人数已经 在过去的十年里，吸烟者使用的香烟越来越多，而且 青少年不仅在消费香烟，而且还在大量消费 无烟烟草。还有人关注的是， 二手烟对非吸烟者的影响，如儿童和青少年 工作场所和家里。由于这些问题，有一个 美国政府正在做出相当大的努力来获得 无烟社会的目标将需要具体的分析 监测和评估这类努力成功与否的程序。 对于特定和敏感的问题，有多种方法可用 烟碱及其主要代谢物可替宁的测定 生物体液。大多数可用的分析方法(如：高效液相、气相色谱、放射免疫分析) 需要昂贵的设备、训练有素的人员和实验室环境。 我们建议开发一种酶免疫分析(EIA)，这将是有用的 用于检测生物体内不同浓度的可替宁 体液。该检测方法可用于监测 烟草产品对儿童发育的影响和对儿童的流行病学研究 吸烟在人群中的地位。该方法简便、快速(<5 分钟)，不需要仪器，因此很容易便携在 工作场所或医生办公室。

The consumption of tobacco products with all its deleterious effects upon the general health of our populace, was and still is a major health problem. Although the number of people consuming cigarettes has decreased over the last decade, smokers are using more cigarettes and adolescents are consuming not only cigarettes but also large quantities of smokeless tobacco. There is also concern with regard to effects of passive smoke on non-smokers such as children and adolescents both in the workplace and in the home. Because of these problems, there is a considerable effort being put forth by the U.S. Government to obtain the goal of a smoke-free society which will require specific analytical procedures to monitor and evaluate the success of such efforts. A variety of methods are available for the specific and sensitive determination of nicotine and its major metabolite, cotinine, in biological fluids. Most of the available assays (i.e., HPLC, GC, RIA) require expensive equipment, trained personnel, and a laboratory setting. We propose to develop an enzyme immunoassay (EIA) which will be useful for the detection of various concentrations of cotinine in biological fluids. This assay can be adapted for use in monitoring the effects of tobacco products on child development and for epidemiological studies on smoking status in the populace. The assay is simple, fast (less than 5 minutes), noninstrumental, and thus easily portable for use in the workplace or physicians office.