FILM SCANNER FOR HIGH THROUGHPUT DNA SEQUENCING

描述（改编自申请人摘要）：总体目标 本研究旨在开发一种高度自动化的DNA测序系统， 可以出售给分子生物学研究人员或感兴趣的商业公司 进行大规模测序项目。 整个系统将 包括机器人反应制备、高容量凝胶电泳 系统、机器人凝胶加载、自动凝胶膜读取器和用于 管理数据。 该提案的具体目标仅限于商业开发， 凝胶盒、放射自显影胶片阅读器和 相关的凝胶组装软件。 目标是一个能够读取多达200，000个原始碱基的系统。 序列（20张X射线胶片，每张10，000个碱基）， 合并以产生20，000到40，000个碱基对的组装序列 每天的吞吐量。 该系统的设计要求至少 注意用户。 据估计，制造的产品将 以低于30，000美元的价格投放市场。

DESCRIPTION (Adapted from applicant's abstract): The overall objective of this research is to develop a highly automated DNA sequencing system that can be sold to molecular biology researchers or commercial firms interested in carrying out large scale sequencing projects. The complete system will include robotic reaction preparation, high capacity gel electrophoresis system, robotic gel loading, automatic gel film reader and software for managing the data. The specific aim of this proposal is limited to development of commercially feasable prototypes of the gel box, the autoradiographic film reader and the associated gel assembly software. The aim is a system with the capability to read up to 200,000 bases of raw sequence per day (20 x-ray films with 10,000 bases each) which can be merged to yield between 20,000 and 40,000 base pairs of assembled sequence per day throughput. The system is designed to require a minimum of attention for the user. It is estimated that the manufactured product will be placed on the market at less than $30,000.