NEW APPROACHES TO FLOW SORTING OF CHROMOSOMES

通过流式分选纯化单个染色体通常是第一步， 这是定义正常和异常人类基因结构的一步。DNA 扩增技术大大减少了分选的量， 所需的材料，使得开发更小，更低成本的可行性 分拣员双光束仪器的性能使用单空气- 冷却氦-镉激光器作为紫外（UV） 和蓝色激发光用于染色染色体的流式细胞术 将评价DAPI/橄榄霉素或Hoechst 33258/色霉素A3， 确定该系统是否可以解析人类核型以及 可以使用现有的商业设备。此外，几个A-T和G-C 偏爱以前未用于染色体分析的DNA荧光染料 并且潜在地可用于大量现有的流分选机， 单光束488或515 nm激发将作为替代方案进行检查， 目前用于双变量流式核型分析的染色剂。

Purification of individual chromosomes by flow sorting is often a first step in defining the structure of normal and abnormal human genes. DNA amplification techniques have greatly reduced the amount of sorted material required, making it feasible to develop smaller, lower-cost sorters. The performance of a dual-beam instrument using a single air- cooled helium-cadmium (He-Cd) laser as a source of both ultraviolet (UV) and blue excitation light for flow Cytometry of chromosomes stained with DAPI/olivomycin or Hoechst 33258/chromomycin A3 will be evaluated to determine whether the system can resolve the human karyotype as well as can existing commercial apparatus. In addition, several A-T and G-C preferring DNA fluorochromes not previously applied to chromosome analysis and potentially usable in a large number of existing flow sorters with single-beam 488 or 515 nm excitation will be examined as alternatives to stains presently used for bivariate flow karyotyping.